Evidence map›Paper›PMID 29573222›Full record

ReviewStem cells translational medicine2018

Concise Review: Altered Versus Unaltered Amniotic Membrane as a Substrate for Limbal Epithelial Cells.

Tor Paaske Utheim, Øygunn Aass Utheim, Panagiotis Salvanos, Catherine J Jackson, Stefan Schrader, Gerd Geerling, Amer Sehic

Abstract readReview
PubMed Full text at PMC Publisher
In one paragraph

Review in Stem cells translational medicine, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed.

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  17. Corneal injury: Clinical and molecular aspects.Experimental eye research · 2019
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Tor Paaske UtheimDepartment of Medical Biochemistry, Oslo University Hospital, Norway.ORCID 0000-0001-8203-5511
Øygunn Aass UtheimDepartment of Ophthalmology, Oslo University Hospital, Norway.
Panagiotis SalvanosDepartment of Ophthalmology, Drammen Hospital, Vestre Viken Hospital Trust, Norway.
Catherine J JacksonDepartment of Medical Biochemistry, Oslo University Hospital, Norway.
Stefan SchraderDepartment of Ophthalmology, University of Düsseldorf, Germany.
Gerd GeerlingDepartment of Ophthalmology, University of Düsseldorf, Germany.
Amer SehicDepartment of Oral Biology, Faculty of Dentistry, University of Oslo, Norway.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Limbal stem cell deficiency (LSCD) can result from a variety of corneal disorders, including chemical and thermal burns, infections, and autoimmune diseases. The symptoms of LSCD may include irritation, epiphora, blepharospasms, photophobia, pain, and decreased vision. There are a number of treatment options, ranging from nonsurgical treatments for mild LSCD to various forms of surgery that involve different cell types cultured on various substrates. Ex vivo expansion of limbal epithelial cells (LEC) involves the culture of LEC harvested either from the patient, a living relative, or a cadaver on a substrate in the laboratory. Following the transfer of the cultured cell sheet onto the cornea of patients suffering from LSCD, a successful outcome can be expected in approximately three out of four patients. The phenotype of the cultured cells has proven to be a key predictor of success. The choice of culture substrate is known to affect the phenotype. Several studies have shown that amniotic membrane (AM) can be used as a substrate for expansion of LEC for subsequent transplantation in the treatment of LSCD. There is currently a debate over whether AM should be denuded (i.e., de-epithelialized) prior to LEC culture, or whether this substrate should remain intact. In addition, crosslinking of the AM has been used to increase the thermal and mechanical stability, optical transparency, and resistance to collagenase digestion of AM. In the present review, we discuss the rationale for using altered versus unaltered AM as a culture substrate for LEC. Stem Cells Translational Medicine 2018;7:415-427.

Indexed as

AmnionAnimalsCell Culture TechniquesCorneal DiseasesEpithelial CellsEpithelium, CornealHumansLimbus CorneaeStem CellsStem Cell TransplantationCell biologyCell transplantationCorneaGene expression

Identifiers

PMID29573222
PMCPMC5905228

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.