ArticleScientific reports2018
Chitosan and cloxacillin combination improve antibiotic efficacy against different lifestyle of coagulase-negative Staphylococcus isolates from chronic bovine mastitis.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 42 citations in OpenAlex.
- Bovine Mastitis Therapy at a Crossroads: Pharmacokinetic Barriers, Biofilms, Antimicrobial Resistance, and Emerging Solutions.Pharmaceuticals (Basel, Switzerland) · 2026Review
- Subclinical mastitis of buffaloes in Asia: prevalence, pathogenesis, risk factors, antimicrobial resistance, and current treatment strategies.Journal of animal science and technology · 2026Review
- Nisin or Chitosan Enhance the Antimicrobial Activity of Ceftiofur Against Antibiotic-ResistantPathogens (Basel, Switzerland) · 2025Article
- Polyhexamethylene Biguanide Nanoparticles Inhibit Biofilm Formation by Mastitis-CausingVeterinary sciences · 2025Article
- Alternative approaches to antibiotics in the control of mastitis in dairy cows: a review.Veterinary research communications · 2025Review
- Antimicrobial Efficacy of Cloxacillin-Loaded Chitosan Nanoparticles Against Staphylococcus aureus Biofilms in Subclinical Mastitis.Current microbiology · 2025Article
- Sublethal concentrations of antibiotics enhance transmission of antibiotic resistance genes in environmentalFrontiers in microbiology · 2025Article
- Quantitative proteomics analysis reveals an important role of the transcriptional regulator UidR in the bacterial biofilm formation ofFrontiers in cellular and infection microbiology · 2024Article
- Intramammary preparation of enrofloxacin hydrochloride-dihydrate for bovine mastitis (biofilm-formingJournal of veterinary science · 2024Article
- Bovine mastitis, a worldwide impact disease: Prevalence, antimicrobial resistance, and viable alternative approaches.Veterinary and animal science · 2023Review
- Advances in Diagnostic Approaches and Therapeutic Management in Bovine Mastitis.Veterinary sciences · 2023Review
- Regulation ofPharmaceutics · 2023Review
- Chitosan can improve antimicrobial treatment independently of bacterial lifestyle, biofilm biomass intensity and antibiotic resistance pattern in non-aureus staphylococci (NAS) isolated from bovine clinical mastitis.Frontiers in microbiology · 2023Article
- Advances in therapeutic and managemental approaches of bovine mastitis: a comprehensive review.The veterinary quarterly · 2021Review
- Antimicrobial activity of polyhexamethylene biguanide nanoparticles against mastitis-causingJDS communications · 2021Article
- Rutin, A Natural Inhibitor of IGPD Protein, Partially Inhibits Biofilm Formation inFrontiers in pharmacology · 2021Article
- Nanoparticles for treatment of bovineDrug delivery · 2020Review
- Synergistic Antifungal Activity of Chitosan with Fluconazole againstMolecules (Basel, Switzerland) · 2020Article
- Inhibition of Drug Resistance ofPolish journal of microbiology · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bovine mastitis affects the health of dairy cows and the profitability of herds worldwide. Coagulase-negative staphylococci (CNS) are the most frequently isolated pathogens in bovine intramammary infection. Based on the wide range of antimicrobial, mucoadhesive and immunostimulant properties demonstrated by chitosan, we have evaluated therapy efficiency of chitosan incorporation to cloxacillin antibiotic as well as its effect against different bacterial lifestyles of seven CNS isolates from chronic intramammary infections. The therapeutic effects of combinations were evaluated on planktonic cultures, bacterial biofilms and intracellular growth in mammary epithelial cells. We found that biofilms and intracellular growth forms offered a strong protection against antibiotic therapy. On the other hand, we found that chitosan addition to cloxacillin efficiently reduced the antibiotic concentration necessary for bacterial killing in different lifestyle. Remarkably, the combined treatment was not only able to inhibit bacterial biofilm establishment and increase preformed biofilm eradication, but it also reduced intracellular bacterial viability while it increased IL-6 secretion by infected epithelial cells. These findings provide a new approach to prophylactic drying therapy that could help to improve conventional antimicrobial treatment against different forms of bacterial growth in an efficient, safer and greener manner reducing multiresistant bacteria generation and spread.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.