ArticleApplied and environmental microbiology2018
Assessment of Passive Traps Combined with High-Throughput Sequencing To Study Airborne Fungal Communities.
Article in Applied and environmental microbiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Metabarcoding Reveals Rich and Diverse Aeromycobiota in Protected Oak Forests.Microbial ecology · 2026Article
- Fungal Spore Richness and Abundance of Allergenic Taxa: Comparing a Portable Impactor and Passive Trap Indoors and Outdoors in an Urban Setting.Microbial ecology · 2024Article
- Development of Novel Early Detection Technology for Hemlock Woolly Adelgid, Adelges tsugae (Hemiptera: Adelgidae).Journal of economic entomology · 2023Article
- In depth search of the Sequence Read Archive database reveals global distribution of the emerging pathogenic fungus Scedosporium aurantiacum.Medical mycology · 2022Article
- Comparison of microscopic and metagenomic approaches to identify cereal pathogens and track fungal spore release in the field.Frontiers in plant science · 2022Article
- Environmental DNA reveals diversity and abundance ofAerobiologia · 2022Article
- Article
- Metagenomics Approaches for the Detection and Surveillance of Emerging and Recurrent Plant Pathogens.Microorganisms · 2021Review
- Monitoring and Surveillance of Aerial Mycobiota of Rice Paddy through DNA Metabarcoding and qPCR.Journal of fungi (Basel, Switzerland) · 2020Article
- Fast and reliable molecular methods to detect fungal pathogens in woody plants.Applied microbiology and biotechnology · 2020Review
- Bioaerosol biomonitoring: Sampling optimization for molecular microbial ecology.Molecular ecology resources · 2019Article
- Mycobiome Diversity in Traditionally Prepared Starters for Alcoholic Beverages in India by High-Throughput Sequencing Method.Frontiers in microbiology · 2019Article
- Article
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4 authors.
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Abstract
Techniques based on high-throughput sequencing (HTS) of environmental DNA have provided a new way of studying fungal diversity. However, these techniques suffer from a number of methodological biases which may appear at any of the steps involved in a metabarcoding study. Air is one of the most important environments where fungi can be found, because it is the primary medium of dispersal for many species. Looking ahead to future developments, it was decided to test 20 protocols, including different passive spore traps, spore recovery procedures, DNA extraction kits, and barcode loci. HTS was performed with the Illumina MiSeq platform targeting two subloci of the fungal internal transcribed spacer. Multivariate analysis and generalized linear models showed that the type of passive spore trap, the spore recovery procedure, and the barcode all impact the description of fungal communities in terms of richness and diversity when assessed by HTS metabarcoding. In contrast, DNA extraction kits did not significantly impact these results. Although passive traps may be used to describe airborne fungal communities, a study using specific real-time PCR and a mock community showed that these kinds of traps are affected by environmental conditions that may induce losses of biological material, impacting diversity and community composition results.
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