Evidence map›Paper›PMID 29570628›Full record

ArticleToxins2018

A Study and Review of Effects of Botulinum Toxins on Mast Cell Dependent and Independent Pruritus.

Roshni Ramachandran, Marc J Marino, Snighdha Paul, Zhenping Wang, Nicholas L Mascarenhas, Sabine Pellett, Eric A Johnson, Anna DiNardo, Tony L Yaksh

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Toxins, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
2.1field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 15 citations in OpenAlex.

  1. Trial
  2. Article
  3. Review
  4. Treatment Options for Troublesome Itch.Pharmaceuticals (Basel, Switzerland) · 2022
    Review
  5. Review
  6. Article
  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Roshni RamachandranDepartment of Anesthesiology, University of California, San Diego, La Jolla, CA 92093, USA. roramachandran@ucsd.edu.
Marc J MarinoDepartment of Anesthesiology, University of California, San Diego, La Jolla, CA 92093, USA. mjmarino@ad.ucsd.edu.
Snighdha PaulDepartment of Anesthesiology, University of California, San Diego, La Jolla, CA 92093, USA. spaul@westernu.edu.
Zhenping WangDepartment of Medicine, Division of Dermatology, University of California, San Diego, La Jolla, CA 92093, USA. zhenping.w@gmail.com.
Nicholas L MascarenhasDepartment of Medicine, Division of Dermatology, University of California, San Diego, La Jolla, CA 92093, USA. nmascare@ucsd.edu.
Sabine PellettDepartment of Bacteriology, University of Wisconsin, Madison, WI 53706, USA. sabine.pellett@wisc.edu.
Eric A JohnsonDepartment of Bacteriology, University of Wisconsin, Madison, WI 53706, USA. eric.johnson@wisc.edu.
Anna DiNardoDepartment of Medicine, Division of Dermatology, University of California, San Diego, La Jolla, CA 92093, USA. adinardo@ucsd.edu.
Tony L YakshDepartment of Anesthesiology, University of California, San Diego, La Jolla, CA 92093, USA. tyaksh@ucsd.edu.
University of California San Diego · USUniversity of Wisconsin–Madison · US

Funding

Construction and Analysis of BoNT derivatives as Pain Specific InhibitorsR21AI113580 · NIAID · UNIVERSITY OF WISCONSIN-MADISON · PI JOHNSON, ERIC A., PELLETT, SABINE · 2014 to 2015
$437k
NIAID NIH HHS R21 AI113580
6 · The paper itself

Abstract

Pruriceptive itch originates following activation of peripheral sensory nerve terminals when pruritogens come in contact with the skin. The ability of botulinum neurotoxins (BoNTs) to attenuate transmitter release from afferent terminals provides a rationale for studying its effect on pruritus. This study investigated the effects of BoNT/A1 and BoNT/B1 on mast cell dependent (Compound 48/80:48/80) and independent (Chloroquine:CQ) scratching. C57Bl/6 male mice received intradermal injection of 1.5 U of BoNT/A1, BoNT/B1 or saline 2, 7, 14 and 21 days prior to ipsilateral 48/80 or CQ at the nape of the neck. Ipsilateral hind paw scratching was determined using an automated recording device. The effect of BoNTs on 48/80 mediated mast cell degranulation was analyzed in human and murine mast cells and the presence of SNAREs was determined using qPCR, immunostaining and Western blot. Pre-treatment with BoNT/A1 and BoNT/B1 reduced 48/80 and CQ induced scratching behavior starting on day 2 with reversal by day 21. Both serotypes inhibited 48/80 induced mast cell degranulation. qPCR and immunostaining detected SNAP-25 mRNA and protein, respectively, in mast cells, however, Western blots did not. This study demonstrates the long-lasting anti-pruritic effects of two BoNT serotypes, in a murine pruritus model using two different mechanistically driven pruritogens. These data also indicate that BoNTs may have a direct effect upon mast cell degranulation.

Indexed as

AnimalsBotulinum Toxins, Type ACell DegranulationCells, CulturedChloroquineHumansMaleMast CellsMice, Inbred C57BLp-Methoxy-N-methylphenethylaminePruritusBotulinum Toxins, Type AChloroquinep-Methoxy-N-methylphenethylaminerimabotulinumtoxinBbotulinum toxinchloroquinecompound 48/80itchmast cellsSNAREVAMP

Identifiers

PMID29570628
PMCPMC5923300
OpenAlexW2792910467

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.