ArticleFASEB journal : official publication of the Federation of American Societies for Experimental Biology2018
Deletion of choline acetyltransferase in enteric neurons results in postnatal intestinal dysmotility and dysbiosis.
Article in FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 40 citations in OpenAlex.
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- Dietary Modulation of the Enteric Nervous System: From Molecular Mechanisms to Therapeutic Applications.Nutrients · 2025Review
- Gut sensory neurons as regulators of neuro-immune-microbial interactions: from molecular mechanisms to precision therapy for IBD/IBS.Journal of neuroinflammation · 2025Review
- Production of Acetylcholine by Podocytes and its Protection from Kidney Injury in GN.Journal of the American Society of Nephrology : JASN · 2025Article
- Nervous system-gut microbiota-immune system axis: future directions for preventing tumor.Frontiers in immunology · 2025Review
- Emerging roles of traditional Chinese medicine in the treatment of diabetic gastroparesis.Frontiers in nutrition · 2025Review
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- Effects of natural products on functional constipation: analysis of active ingredient and mechanism.Naunyn-Schmiedeberg's archives of pharmacology · 2024Review
- Ex Vivo Electrochemical Monitoring of Cholinergic Signaling in the Mouse Colon Using an Enzyme-Based Biosensor.ACS chemical neuroscience · 2023Article
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- Modification of Neurogenic Colonic Motor Behaviours by Chemogenetic Ablation of Calretinin Neurons.Frontiers in cellular neuroscience · 2022Article
- Enteroendocrine cells sense bacterial tryptophan catabolites to activate enteric and vagal neuronal pathways.Cell host & microbe · 2021Article
- An Integrated View on Neuronal Subsets in the Peripheral Nervous System and Their Role in Immunoregulation.Frontiers in immunology · 2021Review
- Electroacupuncture at ST36 Improve the Gastric Motility by Affecting Neurotransmitters in the Enteric Nervous System in Type 2 Diabetic Rats.Evidence-based complementary and alternative medicine : eCAM · 2021Article
- One-to-one relationships between milk miRNA content and protein abundance in neonate duodenum support the potential for milk miRNAs regulating neonate development.Functional & integrative genomics · 2020Article
- Mycotoxins and the Enteric Nervous System.Toxins · 2020Review
Corrections and comments
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Authors and funding
8 authors at 6 institutions in 2 countries.
Funding
Abstract
Acetylcholine (ACh)-synthesizing neurons are major components of the enteric nervous system (ENS). They release ACh and peptidergic neurotransmitters onto enteric neurons and muscle. However, pharmacological interrogation has proven inadequate to demonstrate an essential role for ACh. Our objective was to determine whether elimination of ACh synthesis during embryogenesis alters prenatal viability, intestinal function, the neurotransmitter complement, and the microbiome. Conditional deletion of choline acetyltransferase ( ChAT), the ACh synthetic enzyme, in neural crest-derived neurons ( ChAT-Null) was performed. Survival, ChAT activity, gut motility, and the microbiome were studied. ChAT was conditionally deleted in ENS neural crest-derived cells. Despite ChAT absence, mice were born live and survived the first 2 wk. They failed to gain significant weight in the third postnatal week, dying between postnatal d 18 and 30. Small intestinal transit of carmine red was 50% slower in ChAT-Nulls vs. WT and ChAT- Het. The colons of many neonatal ChAT-Null mice contained compacted feces, suggesting dysmotility. Microbiome analysis revealed dysbiosis in ChAT-Null mice. Developmental deletion of ChAT activity in enteric neurons results in proximal gastrointestinal tract dysmotility, critically diminished colonic transit, failure to thrive, intestinal dysbiosis, and death. ACh is necessary for sustained gut motility and survival of neonatal mice after weaning.-Johnson, C. D., Barlow-Anacker, A. J., Pierre, J. F., Touw, K., Erickson, C. S., Furness, J. B., Epstein, M. L., Gosain, A. Deletion of choline acetyltransferase in enteric neurons results in postnatal intestinal dysmotility and dysbiosis.
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Registered trials
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