ArticlePlant biotechnology journal2018
High-efficiency genome editing using a dmc1 promoter-controlled CRISPR/Cas9 system in maize.
Article in Plant biotechnology journal, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 69 papers.
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Who cites it
69 citing papers in PubMed, 139 citations in OpenAlex.
- A banana susceptibility gene underlying fusarium wilt: validation as a target for disease resistance.Molecular biology reports · 2026Article
- Callus and endosperm green fluorescence reporter-assisted selection system in maize CRISPR/Cas9 gene editing.BMC plant biology · 2025Article
- Decades' progress and prospects on maize functional genomics and molecular breeding.Science China. Life sciences · 2025Review
- High-Frequency Generation of Homozygous/Biallelic Mutants via CRISPR/Cas9 Driven byInternational journal of molecular sciences · 2025Article
- Genome editing in maize and sorghum: A comprehensive review of CRISPR/Cas9 and emerging technologies.The plant genome · 2025Review
- Silencing of disease susceptibility genes: an effective disease resistance strategy against fungal pathogens.Plant cell reports · 2025Review
- Efficient mutagenesis and genotyping of maize inbreds using biolistics, multiplex CRISPR/Cas9 editing, and Indel-Selective PCR.Plant methods · 2025Article
- Improving gene editing of CRISPR/Cas9 using the callus-specific promoterFrontiers in plant science · 2025Article
- Single-stranded DNA (ssDNA) donor repair templates and CRISPR/Cas9 enable a high-frequency of targeted insertions in potato.Frontiers in genome editing · 2025Article
- Development of an endogenous promoter-driven CRISPR/Cas9 system for genome editing inForestry research · 2025Article
- Identification of U6 Promoter and Establishment of Gene-Editing System inPlants (Basel, Switzerland) · 2024Article
- Advancements in Plant Gene Editing Technology: From Construct Design to Enhanced Transformation Efficiency.Biotechnology journal · 2024Review
- Article
- Review
- The potential of genome editing to create novel alleles of resistance genes in rice.Frontiers in genome editing · 2024Review
- An extensible vector toolkit and parts library for advanced engineering of plant genomes.The plant genome · 2023Article
- CRISPR/Cas9 Technology for Potato Functional Genomics and Breeding.Methods in molecular biology (Clifton, N.J.) · 2023Article
- CRISPR/Cas9-mediated homology donor repair base editing confers glyphosate resistance to rice (Frontiers in plant science · 2023Article
- Article
- Transformation and gene editing in the bioenergy grass Miscanthus.Biotechnology for biofuels and bioproducts · 2022Article
9 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 2 institutions in 2 countries.
Funding
Abstract
Previous studies revealed that the promoters for driving both Cas9 and sgRNAs are quite important for efficient genome editing by CRISPR/Cas9 in plants. Here, we report our results of targeted genome editing using the maize dmc1 gene promoter combined with the U3 promoter for Cas9 and sgRNA, respectively. Three loci in the maize genome were selected for targeting. The T0 plants regenerated were highly efficiently edited at the target sites with homozygous or bi-allelic mutants accounting for about 66%. The mutations in T0 plants could be stably transmitted to the T1 generation, and new mutations could be generated in gametes or zygotes. Whole-genome resequencing indicated that no off-target mutations could be detected in the predicted loci with sequence similarity to the targeted site. Our results show that the dmc1 promoter-controlled (DPC) CRISPR/Cas9 system is highly efficient in maize and provide further evidence that the optimization of the promoters used for the CRISPR/Cas9 system is important for enhancing the efficiency of targeted genome editing in plants. The evolutionary conservation of the dmc1 gene suggests its potential for use in other plant species.
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Registered trials
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