ArticleInternational journal of oncology2018
Effects of three-dimensional collagen scaffolds on the expression profiles and biological functions of glioma cells.
Article in International journal of oncology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- Adaptation of Non-Invasive Cancer Cells to 3D Collagen I Microenvironment Induces Transcriptional Reprogramming Accompanied by a More Complex RNA Landscape.International journal of molecular sciences · 2026Article
- Keratocyte Gene Expression Shaped by ECM Dimensionality: Evidence for Enhanced Quiescence in 3D Culture.Bioengineering (Basel, Switzerland) · 2026Article
- Reduced graphene oxide-incorporated gelatin-oxidized polymannose scaffold-based 3D Lung cancer model for mimicking the tumor microenvironment.Frontiers in oncology · 2026Article
- Comparative analysis of 3D culture methodologies in prostate cancer cells.Experimental cell research · 2025Article
- Plasticity of Expression of Stem Cell and EMT Markers in Breast Cancer Cells in 2D and 3D Culture Depend on the Spatial Parameters of Cell Growth; Mathematical Modeling of Mechanical Stress in Cell Culture in Relation to ECM Stiffness.Bioengineering (Basel, Switzerland) · 2025Article
- Development of a hydrogel-based three-dimensional (3D) glioblastoma cell lines culture as a model system for CD73 inhibitor response study.Biomedical engineering online · 2024Article
- Integrated profiling identifies ferredoxin 1 as an immune-related biomarker of malignant phenotype in glioma.Heliyon · 2024Article
- The tumor-associated fibrotic reactions in microenvironment aggravate glioma chemoresistance.Frontiers in oncology · 2024Review
- Gene Expression Profile of 3D Spheroids in Comparison with 2D Cell Cultures and Tissue Strains of Diffuse High-Grade Gliomas.Bulletin of experimental biology and medicine · 2023Article
- Biomaterial-basedCancer pathogenesis and therapy · 2023Review
- Identification of Prominent Genes between 3D Glioblastoma Models and Clinical Samples via GEO/TCGA/CGGA Data Analysis.Biology · 2023Article
- Repairing rat calvarial defects by adipose mesenchymal stem cells and novel freeze-dried three-dimensional nanofibrous scaffolds.BioImpacts : BI · 2023Article
- Three-dimensional in vitro culture models in oncology research.Cell & bioscience · 2022Review
- Review
- Vapor construction and modification of stem cell-laden multicomponent scaffolds for regenerative therapeutics.Materials today. Bio · 2022Article
- Bio-Scaffolds as Cell or Exosome Carriers for Nerve Injury Repair.International journal of molecular sciences · 2021Review
- METTL7B is a novel prognostic biomarker of lower-grade glioma based on pan-cancer analysis.Cancer cell international · 2021Article
- On-chip perivascularLab on a chip · 2021Article
- Identification of collagen genes related to immune infiltration and epithelial-mesenchymal transition in glioma.Cancer cell international · 2021Article
- Review
Corrections and comments
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Authors and funding
17 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Three-dimensional (3D) culture has been increasingly used to investigate tumor cell biology for improved simulation of the natural developing environment. However, the way in which 3D culture affects the gene expression and biological functions of glioma cells remains to be fully elucidated. In the present study, 3D culture environments were established using collagen scaffolds with different pore sizes, followed by the comparison of gene expression profiles and associated biological functions of glioma cells, including the U87, U251 and HS683 cell lines, in 3D collagen scaffolds with conventional two-dimensional (2D) cultured cells. Finally, the possible signaling pathways regulating these differences were investigated. It was found that the 3D collagen scaffold culture upregulated the expression of genes associated with stemness, cell cycle, apoptosis, epithelia-mesenchymal transition, migration, invasion and glioma malignancy, and induced the corresponding functional changes. Apoptotic pathways, the Wnt pathway, Sonic Hedgehog pathway and Notch pathway, may be involved in the regulation of these changes. The aperture size of the collagen-scaffold did not appear to affect the gene expression or functions of the glioma cells. The results of the study suggested that the 3D collagen scaffold enhanced the malignancy of glioma cells and may be a promising in vitro platform for investigations of glioma.
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