ArticleChemical science2017
An acidic pH independent piperazine-TPE AIEgen as a unique bioprobe for lysosome tracing.
Article in Chemical science, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 142 citations in OpenAlex.
- Organelle Localization-Induced Bio-Orthogonal Polymerization (OLIBOP) for Photostable Super-Resolution Live-Cell Imaging.Advanced healthcare materials · 2026Article
- A small-molecule anti-cancer drug for long-acting lysosomal damage.Acta pharmaceutica Sinica. B · 2025Article
- Highly Reversible "On-Off-On" Fluorescence Switch Governed by pH, Utilizing Bis(Benzimidazole) Derivatives with Varied Link Groups.Journal of fluorescence · 2025Article
- A β-galactosidase activated near-infrared fluorescent probe for tracking cellular senescence in vitro and in vivo.Smart molecules : open access · 2025Article
- Photodynamic Therapy: Past, Current, and Future.International journal of molecular sciences · 2024Review
- Organelle-targeting ratiometric fluorescent probes: design principles, detection mechanisms, bio-applications, and challenges.Chemical science · 2023Review
- Dense and Acidic Organelle-Targeted Visualization in Living Cells: Application of Viscosity-Responsive Fluorescence Utilizing Restricted Access to Minimum Energy Conical Intersection.Analytical chemistry · 2023Article
- Fabrication of P and N Co-Doped Carbon Dots for FeBiosensors · 2023Article
- Nanoscale Surface-Enhanced Raman Spectroscopy Investigation of a Polyphenol-Based Plasmonic Nanovector.Nanomaterials (Basel, Switzerland) · 2023Article
- Precision Navigation of Hepatic Ischemia-Reperfusion Injury Guided by Lysosomal Viscosity-Activatable NIR-II Fluorescence.Journal of the American Chemical Society · 2022Article
- Recent progress of surface-enhanced Raman spectroscopy for subcellular compartment analysis.Theranostics · 2021Review
- AIE-based nanoaggregate tracker: high-fidelity visualization of lysosomal movement and drug-escaping processes.Chemical science · 2020Article
- Label-free chlorine and nitrogen-doped fluorescent carbon dots for target imaging of lysosomes in living cells.Mikrochimica acta · 2020Article
- Self-Enhanced Carbonized Polymer Dots for Selective Visualization of Lysosomes and Real-Time Apoptosis Monitoring.iScience · 2020Article
- Article
- Near-infrared fluorescent probes with BODIPY donors and rhodamine and merocyanine acceptors for ratiometric determination of lysosomal pH variance.Sensors and actuators. B, Chemical · 2019Article
- Fluorescent probes for organelle-targeted bioactive species imaging.Chemical science · 2019Review
- Influence of Branched Polyester Chains on the Emission Behavior of Dipyridamole Molecule and Its Biosensing Ability.ACS omega · 2018Article
Corrections and comments
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Authors and funding
13 authors at 3 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lysosomes are involved in a multitude of cellular processes and their dysfunction is associated with various diseases. They are the most acidic organelles (pH 3.8-6.6, size 0.1-1.2 μm) with the highest viscosity (47-190 cP at 25 °C) in the cell. Because of their acidity, pH dependent non-AIE active fluorescent lysosomal probes have been developed that rely on protonation inhibited photoinduced electron transfer (PET). In this work, an acidic pH independent lysosome targetable piperazine-TPE (PIP-TPE) AIEgen has been designed with unique photophysical properties making it a suitable probe for quantifying viscosity. In a non-aggregated state PIP-TPE shows deep-blue emission as opposed to its yellowish-green emission in the bulk. It possesses high specificity for lysosomes with negligible cytotoxicity and good tracing ability due to its better photostability compared to LysoTracker Red. In contrast to most known lysosome probes that rely solely on PET, restriction of intramolecular motion (RIM) due to the larger viscosity inside the lysosomes is the mechanism responsible for PIP-TPE's fluorescence. PIP-TPE's high selectivity is attributed to its unique molecular design that features piperazine fragments providing a perfect balance between lipophilicity and polarity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.