Evidence map›Paper›PMID 29566077›Full record

ArticlePloS one2018

Physiological characterization of leaf and internode after bud break in Japanese indigenous Koshu grape by comparative RNA sequencing analysis.

Shinichi Enoki, Yu Hamaguchi, Shunji Suzuki, Hiroyuki Fujisawa, Tomoki Hattori, Kayo Arita, Chiho Yamaguchi, Masachika Mikami, Shu Nagasaka, Keisuke Tanaka

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In one paragraph

Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Shinichi EnokiLaboratory of Fruit Genetic Engineering, The Institute of Enology and Viticulture, University of Yamanashi, Yamanashi, Japan.
Yu HamaguchiNODAI Genome Research Center, Tokyo University of Agriculture, Tokyo, Japan.
Shunji SuzukiLaboratory of Fruit Genetic Engineering, The Institute of Enology and Viticulture, University of Yamanashi, Yamanashi, Japan.
Hiroyuki FujisawaDepartment of Agriculture, Tokyo University of Agriculture, Kanagawa, Japan.
Tomoki HattoriLaboratory of Fruit Genetic Engineering, The Institute of Enology and Viticulture, University of Yamanashi, Yamanashi, Japan.
Kayo AritaLaboratory of Fruit Genetic Engineering, The Institute of Enology and Viticulture, University of Yamanashi, Yamanashi, Japan.
Chiho YamaguchiLaboratory of Fruit Genetic Engineering, The Institute of Enology and Viticulture, University of Yamanashi, Yamanashi, Japan.
Masachika MikamiLaboratory of Fruit Genetic Engineering, The Institute of Enology and Viticulture, University of Yamanashi, Yamanashi, Japan.
Shu NagasakaLaboratory of Fruit Genetic Engineering, The Institute of Enology and Viticulture, University of Yamanashi, Yamanashi, Japan.
Keisuke TanakaNODAI Genome Research Center, Tokyo University of Agriculture, Tokyo, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Koshu is indigenous to Japan and considered the most important wine grape in Japan. Koshu grape berry possesses characteristics that make it unique from European V. vinifera as wine grape. However, the physiological characteristics of Koshu leaf and internode remain unknown. An understanding of those characteristics would contribute to improvements in Koshu cultivation, thereby enhancing grape berry and wine quality. To identify the genes responsible for the physiological characteristics of Koshu, we comprehensively analyzed leaf and internode differences at the transcriptome level between Koshu and Pinot Noir by RNA sequencing. A total of 248 and 131 differentially expressed genes (DEGs) were detected in leaves and internodes, respectively. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses of these DEGs revealed that "flavonoid biosynthesis" and "glutathione metabolism" pathways were significantly enriched in Koshu leaves. On the other hand, when internodes were compared, "flavonoid"-related GO terms were specifically detected in Koshu. KEGG pathway enrichment analysis suggested that the expression of such genes as leucoanthocyanidin reductase and flavonol synthase in the flavonoid biosynthesis pathway was higher in Koshu than Pinot Noir. Measurement of the relative expression levels of these genes by RT-qPCR validated the results obtained by RNA sequencing. The characteristics of Koshu leaf and internode, which are expected to produce flavonoids with antibacterial activity and UV protection function, would suit Japanese climate as a survival strategy.

Indexed as

FruitGene Expression ProfilingGene Expression Regulation, PlantJapanPlant LeavesSequence Analysis, RNATranscriptomeVitisWine

Identifiers

PMID29566077
PMCPMC5864062

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.