ArticlePloS one2018
Proteomic characterization of fresh spermatozoa and supernatant after cryopreservation in relation to freezability of carp (Cyprinus carpio L) semen.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 30 citations in OpenAlex.
- Proteomic signatures of sperm and extracellular vesicles associated with sperm freezability in Holstein bulls.Scientific reports · 2026Article
- An Overview of Advancements in Proteomic Approaches to Enhance Livestock Production and Aquaculture.Animals : an open access journal from MDPI · 2025Review
- Cryoprotectant-specific alterations in the proteome of Siberian sturgeon spermatozoa induced by cryopreservation.Scientific reports · 2024Article
- Proteomic Insights into Seminal Plasma and Spermatozoa Proteins of Small-Spotted Catsharks,Animals : an open access journal from MDPI · 2024Article
- Type I Diabetes in Zebrafish Reduces Sperm Quality and Increases Insulin and Glucose Transporter Transcripts.International journal of molecular sciences · 2023Article
- DIGE Analysis of Fish Tissues.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Applications of MALDI-MS/MS-Based Proteomics in Biomedical Research.Molecules (Basel, Switzerland) · 2022Review
- Integrated multi-omics analyses reveals molecules governing sperm metabolism potentially influence bull fertility.Scientific reports · 2022Article
- Seminal plasma fractions can protect common carp (Cyprinus carpio) sperm during cryopreservation.Fish physiology and biochemistry · 2020Article
- Freezability biomarkers in bull epididymal spermatozoa.Scientific reports · 2019Article
- Semen cryopreservation for the Mediterranean brown trout of the Biferno River (Molise-Italy): comparative study on the effects of basic extenders and cryoprotectants.Scientific reports · 2019Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Our recent studies suggested that the freezability of carp semen is related to seminal plasma protein profiles. Here, we aimed to compare the spermatozoa proteomes of good (GF) and poor (PF) freezability semen of carp. To achieve this, we used two-dimensional difference in gel electrophoresis followed by MALDI-TOF/TOF mass spectrometry. The semen was classified as GF or PF based on sperm motility after freeze/thawing. We identified proteins enriched in spermatozoa of GF (22 proteins) and PF (18 proteins) semen. We also identified 12 proteins enriched in the supernatant after cryopreservation of PF semen. Good freezability is related to high concentrations of proteins involved in the maintenance of flagella structure, membrane fluidity, efficient control of Ca2+ and sperm motility, energy production, and antioxidative protection, which likely reflects the full maturation status of spermatozoa of GF semen. On the other hand poor freezability seems to be related to the presence of proteins identified as released in high quantities from cryopreserved sperm of PF. Thus, the identified proteins might be useful bioindicators of freezing resilience and could be used to screen carp males before cryopreservation, thus improve long-term sperm preservation in carp. Data are available via ProteomeXchange with identifier PXD008187.
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