Evidence map›Paper›PMID 29564785›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2018

Disruption of Rhodopsin Dimerization in Mouse Rod Photoreceptors by Synthetic Peptides Targeting Dimer Interface.

Sandeep Kumar, Alyssia Lambert, Jon Rainier, Yingbin Fu

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
2.0field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 5 citations in OpenAlex.

  1. GPCR homo-oligomerization.Current opinion in cell biology · 2019
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Sandeep KumarDepartment of Ophthalmology, Baylor College of Medicine, Houston, TX, USA.
Alyssia LambertDepartment of Chemistry, University of Utah, Salt Lake City, UT, USA.
Jon RainierDepartment of Chemistry, University of Utah, Salt Lake City, UT, USA.
Yingbin FuDepartment of Ophthalmology, Baylor College of Medicine, Houston, TX, USA. Yingbin.Fu@bcm.edu.
Baylor College of Medicine · USUniversity of Utah · US

Funding

VISION RESEARCH CENTERP30EY002520 · NEI · BAYLOR COLLEGE OF MEDICINE · PI Samuel M Wu · 1985 to 2026
$14.8M
Cone opsins in photoreceptor degenerationR01EY022614 · NEI · UNIVERSITY OF UTAH · PI FU, YINGBIN · 2012 to 2016
$1.9M
NEI NIH HHS P30 EY002520NEI NIH HHS R01 EY022614
6 · The paper itself

Abstract

Synthetic peptides derived from transmembrane segments of G protein-coupled receptors (GPCR) are used to disrupt GPCR dimer interface. This peptide competition technique is an effective approach to map the dimer interface of GPCR and its functional significance. Here we present a technique to deliver synthetic transmembrane peptides to living mouse rod photoreceptors to disrupt rhodopsin (a prototypical member of Class A GPCRs) dimer formation in the endoplasmic reticulum (ER). We have shown that rhodopsin helix H1- or H8-peptide caused mislocalization of rhodopsin to the perinuclear endoplasmic reticulum (ER).

Indexed as

AnimalsEndoplasmic ReticulumInjections, IntraocularMiceNanoparticlesPeptidesProtein MultimerizationProtein Structure, SecondaryRetinal Rod Photoreceptor CellsRhodopsinPeptidesRhodopsinCone opsinDimer interfaceDimerizationG protein-coupled receptor (GPCR)Nanoparticle deliveryPeptide competitionProtein traffickingRhodopsinRhodopsin dimerRhodopsin helix peptides

Identifiers

PMID29564785
PMCPMC5984041
OpenAlexW2791885991

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.