Evidence map›Paper›PMID 29564372›Full record

ArticleBio-protocol2018

Adapting the Smart-seq2 Protocol for Robust Single Worm RNA-seq.

Lorrayne Serra, Dennis Z Chang, Marissa Macchietto, Katherine Williams, Rabi Murad, Dihong Lu, Adler R Dillman, Ali Mortazavi

Abstract read
In one paragraph

Article in Bio-protocol, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Lorrayne SerraDepartment of Developmental and Cell Biology, University of California, Irvine, California, USA.
Dennis Z ChangDepartment of Nematology, University of California, Riverside, California, USA.
Marissa MacchiettoInstitute of Health Informatics, University of Minnesota, Minneapolis, Minnesota, USA.
Katherine WilliamsDepartment of Developmental and Cell Biology, University of California, Irvine, California, USA.
Rabi MuradDepartment of Developmental and Cell Biology, University of California, Irvine, California, USA.
Dihong LuDepartment of Nematology, University of California, Riverside, California, USA.
Adler R DillmanDepartment of Nematology, University of California, Riverside, California, USA.
Ali MortazaviDepartment of Developmental and Cell Biology, University of California, Irvine, California, USA.

Funding

Comparative analysis of the 4D encoding of regulatory networks in stem cellsDP2GM111100 · NIGMS · UNIVERSITY OF CALIFORNIA-IRVINE · PI MORTAZAVI, SEYED ALI · 2013 to 2013
$2.3M
NIGMS NIH HHS DP2 GM111100
6 · The paper itself

Abstract

Most nematodes are small worms that lack enough RNA for regular RNA-seq protocols without pooling hundred to thousand of individuals. We have adapted the Smart-seq2 protocol in order to sequence the transcriptome of an individual worm. While developed for individual

Indexed as

C. elegansRNA-seqS. carpocapsaeTranscriptome

Identifiers

PMID29564372
PMCPMC5857950

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.