ArticleIranian journal of pathology2017
A Comparative Evaluation of ELISA, PCR, and Serum Agglutination Tests For Diagnosis of Brucella Using Human Serum.
Article in Iranian journal of pathology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 2 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed, 2 syntheses or guidelines pooled it, 37 citations in OpenAlex.
- Prevalence, diagnosis, and manifestations of brucellosis: A systematic review and meta-analysis.Frontiers in veterinary science · 2022Pooled it
- A systematic review and meta-analysis of the prevalence of osteoarticular brucellosis.PLoS neglected tropical diseases · 2019Pooled it
- Comparison of Various Rose Bengal Plate Tests, Serum Tube Agglutination Tests, and Enzyme-Linked Immunosorbent Assays Used in Brucellosis Serosurveillance in Sheep, Bovines, and Spotted Deer.Veterinary sciences · 2026Article
- Establishment of Flow Cytometry-Based Assay for the Quantitative Measurement of Erythrocyte Agglutination in Clinical Laboratory.Indian journal of hematology & blood transfusion : an official journal of Indian Society of Hematology and Blood Transfusion · 2025Article
- Diagnostic Performance of Four Serological Assays for Bovine Brucellosis and Optimised Cutoff Thresholds in an Endemic Region of Iran.Veterinary medicine and science · 2025Article
- Some zoonotic infectious diseases are prevalent among children under 15 years of age in Ardabil Province, northern Iran.BMC infectious diseases · 2025Article
- Brucellosis: Unveiling the complexities of a pervasive zoonotic disease and its global impacts.Open veterinary journal · 2024Review
- Comparative evaluation of RBPT, I-ELISA, and CFT for the diagnosis of brucellosis and PCR detection of Brucella species from Ethiopian sheep, goats, and cattle sera.BMC microbiology · 2023Article
- Evaluating the efficacy of serological testing of clinical specimens collected from patients with suspected brucellosis.PLoS neglected tropical diseases · 2023Article
- Recombinant Omp2b antigen-based ELISA is an efficient tool for specific serodiagnosis of animal brucellosis.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2019Article
- An Overview of Brucellosis in Cattle and Humans, and its Serological and Molecular Diagnosis in Control Strategies.Tropical medicine and infectious disease · 2018Review
- A new way of the Coombs test using flow cytometry-based assay to assess erythrocytes-bound IgG antibodies in the human and rabbit model.International journal of immunopathology and pharmacologyArticle
- Determination of investigation of the link between human and animal Brucella isolates in Iran using multiple-locus variable number tandem repeat method comprising 16 loci (MLVA-16).The Brazilian journal of infectious diseases : an official publication of the Brazilian Society of Infectious DiseasesArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND &
objectiveSince the symptoms of Brucellosis are often atypical and nonspecific, using clinical signs alone to diagnose brucellosis is not advised; therefore, the diagnosis relies predominantly on laboratory testing. Currently, molecular, serological, and microbiological methods are used for diagnosis of this disease. In this study we examined ELISA, PCR and serum agglutination (SAT) methods on human patient serum samples.
methodsA total of 100 serum samples were collected from suspected patients. Fifty serum samples gave a positive result with the Wright test. The ELISA method was first employed on all samples for the detection of IgG and IgM antibodies against
resultsOut of the 100 serum samples obtained from patients with suspected brucellosis, 50 samples tested positive by SAT and displayed high titers of 1/160. Of these 50 positive samples, 49 samples were positive as per the ELISA test whereas one sample tested negative. The PCR test was conducted on all 100 serum samples and results showed that the 45 serum samples that gave a positive agglutination test were also positive by PCR.
conclusionsVarious laboratory methods have been used or introduced for the detection of
Indexed as
Identifiers
29563933PMC5844682W2777835780What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.