ArticleMolecular breeding : new strategies in plant improvement2018
Accelerating public sector rice breeding with high-density KASP markers derived from whole genome sequencing of
Article in Molecular breeding : new strategies in plant improvement, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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42 citing papers in PubMed, 93 citations in OpenAlex.
- Chromosome-level genome of wild-simulated Panax ginseng identifies SNP markers for germplasm and medicinal quality evaluation.Journal of advanced research · 2026Article
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- Integrated BSA-Seq and RNA-Seq reveals genes associated with salt tolerance at the germination stage in foxtail millet (Setaria italica (L.) P. Beauv.).BMC plant biology · 2026Article
- Genetic dissection and validation of major and stable QTLs for the flag leaf size-related traits in common wheat (Triticum aestivum L.).BMC plant biology · 2026Article
- Genomic divergence, introgression and KASP fingerprinting panel development in five cultivar groups of sweet osmanthus.BMC plant biology · 2026Article
- Genome-Wide Development and Validation of KASP-Based SNP Markers inAnimals : an open access journal from MDPI · 2026Article
- Improving nitrogen use efficiency ofFrontiers in plant science · 2026Article
- Development of KASP molecular markers and construction of fingerprinting for cowpea (Vigna unguiculata (L.) Walp.) based on ddRAD-Seq.PloS one · 2026Article
- Development and application of SNP markers to discriminate Korean Perilla (Perilla frutescens) varieties using genomic sequence variations.Scientific reports · 2025Article
- Thousands of trait-specific KASP markers designed for diverse breeding applications in rice (Oryza sativa).G3 (Bethesda, Md.) · 2025Article
- SNP loci identification and KASP marker development system for genetic diversity, population structure, and fingerprinting in sweetpotato (Ipomoea batatas L.).BMC genomics · 2024Article
- Integrated meta-QTL and in silico transcriptome assessment pinpoint major genomic regions responsible for spike length in wheat (Triticum aestivum L.).The plant genome · 2024Article
- Article
- Selection and Validation of 48 KASP Markers for Variety Identification and Breeding Guidance in Conventional and Hybrid Rice (Oryza sativa L.).Rice (New York, N.Y.) · 2022Article
- Efficient marker-assisted breeding for clubroot resistance in elite Pol-CMS rapeseed varieties by updating theMolecular breeding : new strategies in plant improvement · 2022Article
- Potato Cultivar Identification in South Africa Using a Custom SNP Panel.Plants (Basel, Switzerland) · 2022Article
- Molecular sexual determinants in Pistacia genus by KASP assay.Molecular biology reports · 2022Article
- Characterization and QTL Mapping of a Major Field Resistance Locus for Bacterial Blight in Rice.Plants (Basel, Switzerland) · 2022Article
- Applications of Genomic Tools in Plant Breeding: Crop Biofortification.International journal of molecular sciences · 2022Review
- Advances in Understanding and Harnessing the Molecular Regulatory Mechanisms of Vegetable Quality.Frontiers in plant science · 2022Review
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Few public sector rice breeders have the capacity to use NGS-derived markers in their breeding programmes despite rapidly expanding repositories of rice genome sequence data. They rely on > 18,000 mapped microsatellites (SSRs) for marker-assisted selection (MAS) using gel analysis. Lack of knowledge about target SNP and InDel variant loci has hampered the uptake by many breeders of Kompetitive allele-specific PCR (KASP), a proprietary technology of LGC genomics that can distinguish alleles at variant loci. KASP is a cost-effective single-step genotyping technology, cheaper than SSRs and more flexible than genotyping by sequencing (GBS) or array-based genotyping when used in selection programmes. Before this study, there were 2015 rice KASP marker loci in the public domain, mainly identified by array-based screening, leaving large proportions of the rice genome with no KASP coverage. Here we have addressed the urgent need for a wide choice of appropriate rice KASP assays and demonstrated that NGS can detect many more KASP to give full genome coverage. Through re-sequencing of nine
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