ArticleScientific reports2018
Applicability of Automated Cell Counter with a Chlorophyll Detector in Routine Management of Microalgae.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 33 citations in OpenAlex.
- In Situ Determination of Chlorella Concentration Using Single Entity Electrochemistry.Sensors (Basel, Switzerland) · 2026Article
- Validation of an Automated Cell Counter Method for HLA-DR and CD3 Expression in Cells Obtained from Low Volume Human Tears.Diagnostics (Basel, Switzerland) · 2025Article
- The impact of elevated temperature and salinity on microbial communities and food selectivity in heterotrophic nanoflagellates in the Boye River.ISME communications · 2025Article
- Immobilisation of Cellobiose Dehydrogenase and Laccase on Chitosan Particles as a Multi-Enzymatic System for the Synthesis of Lactobionic Acid.Journal of functional biomaterials · 2023Article
- Method for Stress Assessment of Endosymbiotic Algae inMicroorganisms · 2022Article
- Application ofSensors (Basel, Switzerland) · 2021Article
- Activated protein C and PAR1-derived and PAR3-derived peptides are anti-inflammatory by suppressing macrophage NLRP3 inflammasomes.Journal of thrombosis and haemostasis : JTH · 2021Article
- Routine Management of Microalgae Using Autofluorescence from Chlorophyll.Molecules (Basel, Switzerland) · 2019Review
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Authors and funding
1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microalgae have attracted attention for several industrial applications, but all such applications demand culture quality because of their sensitivity to environmental changes. Although simplicity, speed, and accuracy are important to assess algal cultures, researchers have expended vast amounts of labor to monitor algal health using hemocytometry. Along with its user bias, quantifying the cell status aside from the cell density is not easy. This paper describes the easy and rapid evaluation of algal number and status using an image-based cell counter (Countess II FL; Thermo Fisher Scientific Inc.) with a fluorescent filter for chlorophyll. Unlike mammalian cultured cells larger than microalgae, it is not easy for a low-resolution camera alone to distinguish microalgae from grimy spots and microbubbles on counting plates. To assess this method's performance, freshwater/marine microalgae and environmental samples were evaluated using the instrument. Results reveal that an instrument with a fluorescence filter can distinguish microalgae from other particles more precisely than a device with no filter. Values obtained using the instrument were not significantly different from those obtained using hemocytometry. Moreover, the cell counter, but not hemocytometry, can qualify the algal status. Results demonstrate that this system, which has no user bias, can contribute to algal assessment.
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