ArticleScientific reports2018
Impedance-based analysis of Natural Killer cell stimulation.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 23 citations in OpenAlex.
- A Proof-of-Concept Study on Bioelectric-Based Biosensing for Prostate-Specific Antigen Detection in Serum Samples.Biosensors · 2025Article
- Natural killer cell dysfunction is associated with colorectal cancer with severe COVID-19.World journal of gastrointestinal oncology · 2025Article
- Article
- A protocol for quantifying lymphocyte-mediated cytotoxicity using an impedance-based real-time cell analyzer.STAR protocols · 2022Article
- A mechanism of cooling hot tumors: Lactate attenuates inflammation in dendritic cells.iScience · 2021Article
- Biosensors for Detecting Lymphocytes and Immunoglobulins.Biosensors · 2020Review
- Identification of BCL-XL as highly active survival factor and promising therapeutic target in colorectal cancer.Cell death & disease · 2020Article
- Bio-impedance measurement allows displaying the early stages of neutrophil extracellular traps.EXCLI journal · 2020Article
- Article
- NK Cell-Based Glioblastoma Immunotherapy.Cancers · 2018Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The use of impedance-based label free cell analysis is increasingly popular and has many different applications. Here, we report that a real-time cell analyzer (RTCA) can be used to study the stimulation of Natural Killer (NK) cells. Engagement of NK cells via plate-bound antibodies directed against different activating surface receptors could be measured in real time using the label-free detection of impedance. The change in impedance was dependent on early signal transduction events in the NK cells as it was blocked by inhibitors of Src-family kinases and by inhibiting actin polymerization. While CD16 was the only receptor that could induce a strong change in impedance in primary NK cells, several activating receptors induced changes in impedance in expanded NK cells. Using PBMCs we could detect T cell receptor-mediated T cell activation and CD16-mediated NK cell activation in the same sample. Performing a dose-response analysis for the Src-family kinases inhibitor PP1 we show that T cells are more sensitive to inhibition compared to NK cells. Our data demonstrate that the RTCA can be used to detect physiological activation events in NK cells in a label-free and real-time fashion.
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Registered trials
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