ArticleThe Journal of cell biology1987
Assembly of the intestinal brush border: appearance and redistribution of microvillar core proteins in developing chick enterocytes.
Article in The Journal of cell biology, 1987. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 116 citations in OpenAlex.
- Histological Changes of the Mucosal Epithelium in the Chicken Intestine during Pre- and Post-Hatching Stages.The journal of poultry science · 2025Article
- F-actin-bundling sites are conserved in proteins with villin-type headpiece domains.Molecular biology of the cell · 2020Article
- Role of Polarity Proteins in the Generation and Organization of Apical Surface Protrusions.Cold Spring Harbor perspectives in biology · 2018Review
- Restoration of cytoskeletal and membrane tethering defects but not defects in membrane trafficking in the intestinal brush border of mice lacking both myosin Ia and myosin VI.Cytoskeleton (Hoboken, N.J.) · 2015Article
- Villin and actin in the mouse kidney brush-border membrane bind to and are degraded by meprins, an interaction that contributes to injury in ischemia-reperfusion.American journal of physiology. Renal physiology · 2011Article
- Regulation of cell structure and function by actin-binding proteins: villin's perspective.FEBS letters · 2008Review
- Parallel actin bundles and their multiple actin-bundling proteins.Current opinion in cell biology · 2000Review
- F-actin bundles are derivatives of microvilli: What does this tell us about how bundles might form?The Journal of cell biology · 2000Review
- Why are two different cross-linkers necessary for actin bundle formation in vivo and what does each cross-link contribute?The Journal of cell biology · 1998Article
- The apical submembrane cytoskeleton participates in the organization of the apical pole in epithelial cells.The Journal of cell biology · 1997Article
- Dynamic effects of acid on Barrett's esophagus. An ex vivo proliferation and differentiation model.The Journal of clinical investigation · 1996Article
- F actin bundles in Drosophila bristles. I. Two filament cross-links are involved in bundling.The Journal of cell biology · 1995Article
- Suppression of villin expression by antisense RNA impairs brush border assembly in polarized epithelial intestinal cells.The EMBO journal · 1995Article
- Molecular motors are differentially distributed on Golgi membranes from polarized epithelial cells.The Journal of cell biology · 1994Article
- Localization of brush border cytoskeletal proteins in gastric oxynticopeptic cells from the bullfrog Rana catesbeiana.Cell and tissue research · 1994Article
- Golgi-derived vesicles from developing epithelial cells bind actin filaments and possess myosin-I as a cytoplasmically oriented peripheral membrane protein.The Journal of cell biology · 1993Article
- Changes in keratin expression during fetal and postnatal development of intestinal epithelial cells.The Biochemical journal · 1992Article
- Microinjection of villin into cultured cells induces rapid and long-lasting changes in cell morphology but does not inhibit cytokinesis, cell motility, or membrane ruffling.The Journal of cell biology · 1990Article
- Binding of brush border myosin I to phospholipid vesicles.The Journal of cell biology · 1990Article
- Fodrin as a differentiation marker. Redistributions in colonic neoplasia.The American journal of pathology · 1989Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The assembly of the intestinal microvillus cytoskeleton during embryogenesis in the chick was examined by immunochemical and light microscopic immunolocalization techniques. For these studies, affinity-purified antibodies reactive with three major cytoskeletal proteins of the adult intestinal microvillus, fimbrin, villin, and the 110-kD subunit of the 110K-calmodulin protein complex were prepared. Immunocytochemical staining of frozen sections of embryonic duodena revealed that all three proteins were present at detectable levels at the earliest stages examined, day 7-8 of incubation (Hamilton/Hamburger stages 25-30). Although initially all three proteins were diffusely distributed throughout the cytoplasm, there was a marked asynchrony in the accumulation of these core proteins within the apical domain of the enterocyte. Villin displayed concentrated apical staining by embryonic day 8 (stage 28), while the apical concentration of fimbrin was first observed at embryonic day 10 (stage 37). Diffuse staining of the enterocyte cytoplasm with the anti-110K was observed throughout development until a few days before hatch. By embryonic day 19-21 110K staining was concentrated at the cell periphery (apical and basolateral). The restricted apical localization characteristic of 110K in the adult brush border was not observed until the day of hatching. Immunoblot analysis of whole, solubilized embryonic duodena confirmed the presence of 110K, villin, and fimbrin throughout development and indicated substantial increases in all three proteins, particularly late in development. Immunoblot staining with anti-110K also revealed the presence of a high molecular mass (200 kD) immunoreactive species in embryonic intestine. This 200-kD form was absent from isolated embryonic enterocytes and may be a component of intestinal smooth muscle.
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