ArticleChemical science2015
Enzyme-mediated single-nucleotide variation detection at room temperature with high discrimination factor.
Article in Chemical science, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed, 58 citations in OpenAlex.
- Heteromultivalency enables enhanced detection of nucleic acid mutations.Nature chemistry · 2024Article
- Enhanced SNP-sensing using DNA-templated reactions through confined hybridization of minimal substrates (CHOMS).Chemical science · 2020Article
- Detection of KRAS mutation via ligation-initiated LAMP reaction.Scientific reports · 2019Article
- Exonuclease III-Regulated Target Cyclic Amplification-Based Single Nucleotide Polymorphism Detection Using Ultrathin Ternary Chalcogenide Nanosheets.Frontiers in chemistry · 2019Article
- Endonuclease IV recognizes single base mismatch on the eighth base 3' to the abasic site in DNA strands for ultra-selective and sensitive mutant-type DNA detection.RSC advances · 2018Article
- Simultaneous multiple single nucleotide polymorphism detection based on click chemistry combined with DNA-encoded probes.Chemical science · 2018Article
- Noncanonical substrate preference of lambda exonuclease for 5'-nonphosphate-ended dsDNA and a mismatch-induced acceleration effect on the enzymatic reaction.Nucleic acids research · 2018Article
- DNA terminal structure-mediated enzymatic reaction for ultra-sensitive discrimination of single nucleotide variations in circulating cell-free DNA.Nucleic acids research · 2018Article
- A branch-migration based fluorescent probe for straightforward, sensitive and specific discrimination of DNA mutations.Nucleic acids research · 2017Article
- Single-Molecule Counting of Point Mutations by Transient DNA Binding.Scientific reports · 2017Article
- Ultra-specific discrimination of single-nucleotide mutations using sequestration-assisted molecular beacons.Chemical science · 2017Article
- A universal probe design for colorimetric detection of single-nucleotide variation with visible readout and high specificity.Scientific reports · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We demonstrate a new powerful tool to detect single-nucleotide variation in DNA at room temperature with high selectivity, based on predetermined specific interactions between Lambda exonuclease and a chemically modified DNA substrate structure which comprises two purposefully introduced mismatches and a covalently attached fluorophore. The fluorophore not only acts as a signal reporter in the detection system, but also plays a notable role in the specific molecular recognition between the enzyme and the probe/target hybrid substrate. The method is single-step, rapid, and can be easily adapted to different high-throughput micro-devices without the need for temperature control.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.