Article3 Biotech2018
A rapid and efficient SDS-based RNA isolation protocol from different tissues of coffee.
Article in 3 Biotech, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 13 citations in OpenAlex.
- Optimized SDS-Based Protocol for High-Quality RNA Extraction fromMethods and protocols · 2025Article
- Genome-wide analysis and identification of Carotenoid Cleavage Oxygenase (CCO) gene family in coffee (coffee arabica) under abiotic stress.BMC genomic data · 2024Article
- Comparative genomic analysis and phylogeny of NAC25 gene from cultivated and wildFrontiers in plant science · 2022Article
- Improved method for isolation of high-quality total RNA from3 Biotech · 2021Article
- Two-step method for isolation of high-quality RNA from stored seeds of maize rich in starch.3 Biotech · 2020Article
- High-quality RNA isolation from pigment-rich3 Biotech · 2019Article
- A phenol/chloroform-free method to extract nucleic acids from recalcitrant, woody tropical species for gene expression and sequencing.Plant methods · 2019Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Isolation of high-quality RNA from coffee is challenging because of high level of polysaccharides, polyphenols and other secondary metabolites. In the present study, a rapid and efficient RNA extraction protocol from different tissues of coffee was optimized. Sufficiently high quality and quantity (225.6-454.8 µg/g) of RNA was obtained by using the optimized protocol. The presence of two distinct bands of 28S rRNA and 18S rRNA in agarose gel proved the intactness of the RNA samples. The average spectrophotometric values of the isolated RNA ranged from 1.96 to 2.02 (
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