ArticleBiomaterials research2018
The effect of serum types on Chondrogenic differentiation of adipose-derived stem cells.
Article in Biomaterials research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Adipose-mesenchymal stem cells enhance the formation of auricular cartilage in vitro and in vivo.Stem cells translational medicine · 2025Article
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- In vivo imaging of adipose-derived stem cell sheets on biodegradable nonwoven fabric using X-ray CT.Biomedical engineering online · 2024Article
- Human gelatin-based composite hydrogels for osteochondral tissue engineering and their adaptation into bioinks for extrusion, inkjet, and digital light processing bioprinting.Biofabrication · 2022Article
- Synovial fluid niche promoted differentiation of dental follicle mesenchymal stem cells toward chondrogenesis in rheumatoid arthritis.Archives of rheumatology · 2022Article
- Articular Cartilage Regeneration in Veterinary Medicine.Advances in experimental medicine and biology · 2022Article
- 'Fat chance': a review of adipose tissue engineering and its role in plastic and reconstructive surgery.Annals of the Royal College of Surgeons of England · 2021Review
- Bioconversion Products of Whey by Lactic Acid Bacteria Exert Anti-Adipogenic Effect.Food science of animal resources · 2021Review
- A comparison of the effects of fetal bovine serum and newborn calf serum on cell growth and maintenance of cryopreserved mouse spermatogonial stem cells.Molecular biology reports · 2020Article
- Extracellular matrix-based biomaterials as adipose-derived stem cell delivery vehicles in wound healing: a comparative study between a collagen scaffold and two xenografts.Stem cell research & therapy · 2020Article
- Chondrogenic Differentiation of Pluripotent Stem Cells under Controllable Serum-Free Conditions.International journal of molecular sciences · 2019Article
- In Vitro Effect of Estradiol and Progesterone on Ovine Amniotic Epithelial Cells.Stem cells international · 2019Article
- A Non-Enzymatic Method to Obtain a Fat Tissue Derivative Highly Enriched in Adipose Stem Cells (ASCs) from Human Lipoaspirates: Preliminary Results.International journal of molecular sciences · 2018Article
- Induction of chondrogenesis of human placenta-derived mesenchymal stem cells via heparin-grafted human fibroblast derived matrix.Biomaterials research · 2018Article
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3 authors.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundFetal bovine serum (FBS) is the most essential supplement in culture media for cellular proliferation, metabolism, and differentiation. However, due to a limited supply and subsequently rising prices, a series of studies have investigated a biological feasibility of replaceable serums to substitute FBS. Along with the increasing interests to manufacture stem cell-based cellular products, optimizing the composition of culture media including serums and exogenous growth factors (GFs) is of importance. In this experiment, the effect of bovine serum (BS) and newborn calf serum (NCS) on proliferation and chondrogenic differentiation capacity of human adipose derived stem cells (ADSCs) was evaluated, especially in the chondrogenically supplemented culture condition.
methodsADSCs were chondrogenically cultured with FBS, BS, and NCS for 14 days. For the acceleration of in vitro chondrogenesis, exogenous insulin-like growth factor and transforming growth factor-β3 were added. Viability and proliferation of ADSCs were evaluated using Live/Dead fluorescence staining and DNA amount, respectively. To investigate a chondrogenic differentiation, a series of assays were performed including a quantification of glycosaminoglycan deposition, alcian blue staining, and RT-PCR analysis for type II collagen, aggrecan and Sox-9 genes.
resultsThe results demonstrated that proliferation of ADSCs was facilitated in FBS condition as compared with other serum types. For chondrogenic marker gene expression, serum substitutes enhanced Sox-9 expression level on day 14. The deposition of glycosaminoglycan was more facilitated in BS condition regardless of additional chondrogenic GFs.
conclusionIt could be presumably speculated that serum types and exogenous supplements of GFs could also be important parameters to optimize culture media composition, especially in order to maintain the enhanced levels of both proliferation and chondrogenic differentiation of ADSCs during expansion.
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