Evidence map›Paper›PMID 29555787›Full record

ArticlePlant physiology2018

Multiplex Fluorescent, Activity-Based Protein Profiling Identifies Active α-Glycosidases and Other Hydrolases in Plants.

Amjad M Husaini, Kyoko Morimoto, Balakumaran Chandrasekar, Steven Kelly, Farnusch Kaschani, Daniel Palmero, Jianbing Jiang, Markus Kaiser, Oussama Ahrazem, Hermen S Overkleeft and 1 more

Open access · hybridAbstract read
In one paragraph

Article in Plant physiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
3.0field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed, 32 citations in OpenAlex.

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  14. Proteomic Analysis of Mycelial Exudates ofPathogens (Basel, Switzerland) · 2021
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 6 institutions in 5 countries.

Amjad M HusainiDepartment of Plant Sciences, University of Oxford, Oxford OX1 3RB, United Kingdom.ORCID 0000-0003-1293-3118
Kyoko MorimotoDepartment of Plant Sciences, University of Oxford, Oxford OX1 3RB, United Kingdom.
Balakumaran ChandrasekarDepartment of Plant Sciences, University of Oxford, Oxford OX1 3RB, United Kingdom.
Steven KellyDepartment of Plant Sciences, University of Oxford, Oxford OX1 3RB, United Kingdom.
Farnusch KaschaniChemische Biologie, Zentrum für Medizinische Biotechnologie, Fakultät für Biologie, Universität Duisburg-Essen, 45117 Essen, Germany.
Daniel PalmeroUniversidad Politécnica de Madrid, Escuela Técnica Superior de Ingenieria Agronómica, Alimentaria cv de Biosistemas, Ciudad Universitaria s/n, 28040 Madrid, Spain.ORCID 0000-0002-1442-1274
Jianbing JiangGorlaeus Laboratories, Leiden Institute of Chemistry and Netherlands Center for Proteomics, 2333 CC Leiden, The Netherlands.ORCID 0000-0003-4792-8140
Markus KaiserChemische Biologie, Zentrum für Medizinische Biotechnologie, Fakultät für Biologie, Universität Duisburg-Essen, 45117 Essen, Germany.
Oussama AhrazemInstituto Botánico, Facultad de Farmacia, Universidad de Castilla-La Mancha, Campus Universitario s/n, Albacete 02071, Spain.ORCID 0000-0001-9183-9319
Hermen S OverkleeftGorlaeus Laboratories, Leiden Institute of Chemistry and Netherlands Center for Proteomics, 2333 CC Leiden, The Netherlands.
Renier A L van der HoornDepartment of Plant Sciences, University of Oxford, Oxford OX1 3RB, United Kingdom renier.vanderhoorn@plants.ox.ac.uk.ORCID 0000-0002-3692-7487
University of Oxford · GBNetherlands Metabolomics Centre · NLUniversity of Duisburg-Essen · DESher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir · INUniversidad Politécnica de Madrid · ESUniversity of Castilla-La Mancha · ES

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

With nearly 140 α-glycosidases in 14 different families, plants are well equipped with enzymes that can break the α-glucosidic bonds in a large diversity of molecules. Here, we introduce activity-based protein profiling (ABPP) of α-glycosidases in plants using α-configured cyclophellitol aziridine probes carrying various fluorophores or biotin. In Arabidopsis (

Indexed as

AcarboseArabidopsis ProteinsBiotinylationCarbocyaninesCatalytic DomainCrocusEnzyme InhibitorsFluorescent DyesFusariumGalactosamineGlucosidasesGlycoside HydrolasesHydrogen-Ion ConcentrationPlant DiseasesPlant ProteinsProteomics5-amino-5-deoxygalactopyranoseAcarboseArabidopsis ProteinsCarbocyaninescyanine dye 5Enzyme InhibitorsFluorescent DyesGalactosamineGlucosidasesGlycoside HydrolasesPlant Proteins

Identifiers

PMID29555787
PMCPMC5933116
OpenAlexW2790190019

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.