ArticleProceedings of the National Academy of Sciences of the United States of America2018
Live births from artificial insemination of microfluidic-sorted bovine spermatozoa characterized by trajectories correlated with fertility.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 82 citations in OpenAlex.
- Sperm Recovery and Evaluation in Domestic Species: Challenges and Current Practices.Reproduction in domestic animals = Zuchthygiene · 2026Review
- Establishment and in vitro validation of a sheep-specific, low-cost TLR7/8 agonist R848-mediated X/Y sperm separation system.BMC biotechnology · 2026Article
- Evaluation of the Feasibility of a Microfluidic Method Using the ZYMŌT System for Canine Sperm Selection.Reproduction in domestic animals = Zuchthygiene · 2026Article
- Microfluidics for in vitro fertilization: from science to clinical validation.Human reproduction update · 2026Review
- Let the female tract choose: finding the sperm to enhanceAnimal reproduction · 2026Article
- Simple and rapid microfluidic device for processing raw human semen and separation of motile sperm based on their intrinsic behavior and morphology.Scientific reports · 2025Article
- Assessing Bovine Male Fertility in a Technological Age.Advances in anatomy, embryology, and cell biology · 2025Review
- Sperm cellular and nuclear dynamics associated with ram fertility.Frontiers in veterinary science · 2025Article
- Microfluidics-A novel technique for high-quality sperm selection for greater ART outcomes.FASEB bioAdvances · 2024Review
- Effect of Two Different Sperm Selection Methods on Boar Sperm Parameters and In Vitro Fertilisation Outcomes.Animals : an open access journal from MDPI · 2024Article
- Advances in microfluidic technology for sperm screening and in vitro fertilization.Analytical and bioanalytical chemistry · 2024Review
- Qualified sperm selection based on the rheotaxis and thigmotaxis in a microfluidic system.Biomedical engineering letters · 2023Article
- Truncating PICK1 Variant Identified in Azoospermia Affected Mitochondrial Dysfunction in Knockout Mice.Current medical science · 2023Article
- Opportunities involving microfluidics and 3D culture systems to theAnimal reproduction · 2023Review
- Developing biomedical engineering technologies for reproductive medicine.Smart medicine · 2022Review
- Recent Advancements in In Vitro Fertilisation.Cureus · 2022Review
- New Approaches to Define The Functional Competency of Human Sperm Subpopulations and Its Relationship to Semen Quality.International journal of fertility & sterility · 2022Article
- Metal deposition and shape reproduction at biological temperatures on cell-level samples.Scientific reports · 2022Article
- Review
- The Future of IVF: The New Normal in Human Reproduction.Reproductive sciences (Thousand Oaks, Calif.) · 2022Article
Corrections and comments
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Authors and funding
16 authors at 8 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Selection of functional spermatozoa plays a crucial role in assisted reproduction. Passage of spermatozoa through the female reproductive tract requires progressive motility to locate the oocyte. This preferential ability to reach the fertilization site confers fertility advantage to spermatozoa. Current routine sperm selection techniques are inadequate and fail to provide conclusive evidence on the sperm characteristics that may affect fertilization. We therefore developed a selection strategy for functional and progressively motile bovine spermatozoa with high DNA integrity based on the ability to cross laminar flow streamlines in a diffuser-type microfluidic sperm sorter (DMSS). The fluid dynamics, with respect to microchannel geometry and design, are relevant in the propulsion of spermatozoa and, consequently, ultrahigh-throughput sorting. Sorted spermatozoa were assessed for kinematic parameters, acrosome reaction, mitochondrial membrane potential, and DNA integrity. Kinematic and trajectory patterns were used to identify fertility-related subpopulations: the rapid, straighter, progressive, nonsinuous pattern (PN) and the transitional, sinuous pattern (TS). In contrast to the conventional notion that the fertilizing spermatozoon is always vigorously motile and more linear, our results demonstrate that sinuous patterns are associated with fertility and correspond to truly functional spermatozoa as supported by more live births produced from predominant TS than PN subpopulation in the inseminate. Our findings ascertain the true practical application significance of microfluidic sorting of functional sperm characterized by sinuous trajectories that can serve as a behavioral sperm phenotype marker for fertility potential. More broadly, we foresee the clinical application of this sorting technology to assisted reproduction in humans.
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