ArticleDevelopmental biology2018
Cdc42 controls primary mesenchyme cell morphogenesis in the sea urchin embryo.
Article in Developmental biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 30 citations in OpenAlex.
- ROCK and the actomyosin network control biomineral growth and morphology during sea urchin skeletogenesis.eLife · 2024Article
- microRNA-1 regulates sea urchin skeletogenesis by directly targeting skeletogenic genes and modulating components of signaling pathways.Developmental biology · 2024Article
- Transcription of microRNAs is regulated by developmental signaling pathways and transcription factors.Frontiers in cell and developmental biology · 2024Article
- Micromere formation and its evolutionary implications in the sea urchin.Current topics in developmental biology · 2022Article
- Use of Echinoderm Gametes and Early Embryos for Studying Meiosis and Mitosis.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Synthesis, Screening and Characterization of Novel Potent Arp2/3 Inhibitory Compounds Analogous to CK-666.Frontiers in pharmacology · 2022Article
- Live imaging of echinoderm embryos to illuminate evo-devo.Frontiers in cell and developmental biology · 2022Article
- Rab35 regulates skeletogenesis and gastrulation by facilitating actin remodeling and vesicular trafficking.Cells & development · 2021Article
- Calcium-vesicles perform active diffusion in the sea urchin embryo during larval biomineralization.PLoS computational biology · 2021Article
- Review
- Rac and Arp2/3-Nucleated Actin Networks Antagonize Rho During Mitotic and Meiotic Cleavages.Frontiers in cell and developmental biology · 2020Article
- Cytoskeletal polarization and cytokinetic signaling drives polar lobe formation in spiralian embryos.Developmental biology · 2019Article
- Possible cooption of a VEGF-driven tubulogenesis program for biomineralization in echinoderms.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Culture of and experiments with sea urchin embryo primary mesenchyme cells.Methods in cell biology · 2019Review
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Authors and funding
4 authors at 2 institutions in 1 country.
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Abstract
In the sea urchin embryo, gastrulation is characterized by the ingression and directed cell migration of primary mesenchyme cells (PMCs), as well as the primary invagination and convergent extension of the endomesoderm. Like all cell shape changes, individual and collective cell motility is orchestrated by Rho family GTPases and their modulation of the actomyosin cytoskeleton. And while endomesoderm specification has been intensively studied in echinoids, much less is known about the proximate regulators driving cell motility. Toward these ends, we employed anti-sense morpholinos, mutant alleles and pharmacological inhibitors to assess the role of Cdc42 during sea urchin gastrulation. While inhibition of Cdc42 expression or activity had only mild effects on PMC ingression, PMC migration, alignment and skeletogenesis were disrupted in the absence of Cdc42, as well as elongation of the archenteron. PMC migration and patterning of the larval skeleton relies on the extension of filopodia, and Cdc42 was required for filopodia in vivo as well as in cultured PMCs. Lastly, filopodial extension required both Arp2/3 and formin actin-nucleating factors, supporting models of filopodial nucleation observed in other systems. Together, these results suggest that Cdc42 plays essential roles during PMC cell motility and organogenesis.
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