Evidence map›Paper›PMID 29554889›Full record

ArticleBMC molecular biology2018

pH-mediated upregulation of AQP1 gene expression through the Spi-B transcription factor.

Yihui Zhai, Hong Xu, Qian Shen, Franz Schaefer, Claus P Schmitt, Jing Chen, Haimei Liu, Jialu Liu, Jiaojiao Liu

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC molecular biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.4field-weighted citation impact, top 40% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 2 countries.

Yihui ZhaiDepartment of Nephrology and Rheumatology, Children's Hospital of Fudan University, No. 399 Wanyuan Road, Shanghai, 201102, China.
Hong XuDepartment of Nephrology and Rheumatology, Children's Hospital of Fudan University, No. 399 Wanyuan Road, Shanghai, 201102, China. hxu@shmu.edu.cn.
Qian ShenDepartment of Nephrology and Rheumatology, Children's Hospital of Fudan University, No. 399 Wanyuan Road, Shanghai, 201102, China.
Franz SchaeferDivision of Pediatric Nephrology, Center for Pediatric and Adolescent Medicine, University of Heidelberg, Heidelberg, Germany.
Claus P SchmittDivision of Pediatric Nephrology, Center for Pediatric and Adolescent Medicine, University of Heidelberg, Heidelberg, Germany.
Jing ChenDepartment of Nephrology and Rheumatology, Children's Hospital of Fudan University, No. 399 Wanyuan Road, Shanghai, 201102, China.
Haimei LiuDepartment of Nephrology and Rheumatology, Children's Hospital of Fudan University, No. 399 Wanyuan Road, Shanghai, 201102, China.
Jialu LiuDepartment of Nephrology and Rheumatology, Children's Hospital of Fudan University, No. 399 Wanyuan Road, Shanghai, 201102, China.
Jiaojiao LiuDepartment of Nephrology and Rheumatology, Children's Hospital of Fudan University, No. 399 Wanyuan Road, Shanghai, 201102, China.
Children's Hospital of Fudan University · CNHeidelberg University · DE

Funding

National Natural Science Foundation of China 81300635Shanghai Natural Science Fund for Youth Scholars 12ZR1441300Young Teachers Foundation Project supported by the Doctorate in Higher Education Institutions of Ministry of Education 20120071120096
6 · The paper itself

Abstract

backgroundBicarbonate-based peritoneal dialysis (PD) fluids enhance the migratory capacity and damage-repair ability of human peritoneal mesothelial cells by upregulating AQP1. However, little is known about the underlying molecular mechanisms.

resultsHere we used HEK-293T cells to investigate the effect of pH on AQP1 gene transcription levels. We found that AQP1 mRNA levels increases with pH. Transfection of HEK-293T cells with luciferase reporter vectors containing different regions of the AQP1 promoter identified an upstream region in the AQP1 gene between - 2200 and - 2300 bp as an enhancer required for pH-mediated regulation of AQP1 expression. Site-directed mutagenesis of this specific promoter region revealed a critical region between - 2257 and - 2251 bp, and gene knock-down experiments and ChIP assays suggested that the Spi-B transcription factor SPIB is involved in pH-mediated regulation of AQP1 expression.

conclusionsWe identified an upstream region in the AQP1 gene and the transcription factor SPIB that are critically involved in pH-mediated regulation of AQP1 expression. These findings provide the basis for further studies on the pH- and buffer-dependent effects of PD fluids on peritoneal membrane integrity and function.

Indexed as

Up-RegulationAquaporin 1Binding SitesDNA-Binding ProteinsGene Knockdown TechniquesHEK293 CellsHumansHydrogen-Ion ConcentrationMutagenesis, Site-DirectedPromoter Regions, GeneticTranscription FactorsAQP1 protein, humanAquaporin 1DNA-Binding ProteinsSPIB protein, humanTranscription FactorsAquaporin 1Peritoneal dialysis fluidspHPromoterTranscription

Identifiers

PMID29554889
PMCPMC5859780
OpenAlexW2802654955

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.