ArticlePloS one2018
Assessment of Aedes albopictus reference genes for quantitative PCR at different stages of development.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Article
- Development of a Novel Immune Gene Array to Assess Mosquito Gene Expression During Arbovirus Infection.Viruses · 2026Article
- Stability and suitability of housekeeping genes in phlebotomine sand flies.Scientific reports · 2024Article
- Light pollution disrupts circadian clock gene expression in two mosquito vectors during their overwintering dormancy.Scientific reports · 2024Article
- Reference Genes for Expression Analysis Using RT-qPCR inInsects · 2022Article
- The Dengue Virus Nonstructural Protein 1 (NS1) Interacts with the Putative Epigenetic Regulator DIDO1 to Promote Flavivirus Replication in Mosquito Cells.Journal of virology · 2022Article
- Temperate Conditions Limit Zika Virus Genome Replication.Journal of virology · 2022Article
- A species-specific lncRNA modulates the reproductive ability of the asian tiger mosquito.Frontiers in bioengineering and biotechnology · 2022Article
- A comparative analysis ofCurrent research in insect science · 2022Article
- Genome-wide identification of Aedes albopictus long noncoding RNAs and their association with dengue and Zika virus infection.PLoS neglected tropical diseases · 2021Article
- Selection of Reference Genes for Quantitative Real-Time PCR inFrontiers in physiology · 2020Article
- Identification and Evaluation of Reference Genes for Normalization of Gene Expression in Developmental Stages, Sexes, and Tissues of Diaphania caesalis (Lepidoptera, Pyralidae).Journal of insect science (Online) · 2020Article
- RNA interference-mediated knockdown of 3, 4-dihydroxyphenylacetaldehyde synthase affects larval development and adult survival in the mosquito Aedes aegypti.Parasites & vectors · 2019Article
- Fat body-specific vitellogenin expression regulates host-seeking behaviour in the mosquito Aedes albopictus.PLoS biology · 2019Article
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2 authors.
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Abstract
Members of the Aedes genus of mosquitoes are widely recognized as vectors of viral diseases. Ae.albopictus is its most invasive species, and are known to carry viruses such as Dengue, Chikugunya and Zika. Its emerging importance puts Ae.albopictus on the forefront of genetic interaction and evolution studies. However, a panel of suitable reference genes specific for this insect is as of now undescribed. Nine reference genes, namely ACT, eEF1-γ, eIF2α, PP2A, RPL32, RPS17, PGK1, ILK and STK were evaluated. Expression patterns of the candidate reference genes were observed in a total of seventeen sample types, separated by stage of development and age. Gene stability was inferred from obtained quantification data through three widely cited evaluation algorithms i.e. BestKeeper, geNorm, and NormFinder. No single gene showed a satisfactory degree of stability throughout all developmental stages. Therefore, we propose combinations of PGK and ILK for early embryos; RPL32 and RPS17 for late embryos, all four larval instars, and pupae samples; eEF1-γ with STK for adult males; eEF1-γ with RPS17 for non-blood fed females; and eEF1-γ with eIF2α for both blood-fed females and cell culture. The results from this study should be able to provide a more informed selection of normalizing genes during qPCR in Ae.albopictus.
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