ArticleJournal of visualized experiments : JoVE2018
Quantification of Lipid Abundance and Evaluation of Lipid Distribution in Caenorhabditis elegans by Nile Red and Oil Red O Staining.
Article in Journal of visualized experiments : JoVE, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 86 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
86 citing papers in PubMed.
- Neuropeptides NLP-10 and FLP-18 stimulate lipid catabolism in chronically diet-restrictedbioRxiv : the preprint server for biology · 2026Article
- Bovine viral diarrhea virus (BVDV) relies on cellular lipid droplet biogenesis and lipolysis to provide energy for viral replication.Veterinary research · 2026Article
- Fasting requires Peroxiredoxin 2 and Peroxiredoxin 6 to coordinate redox dependent mitochondrial and lipid remodelling in Caenorhabditis elegans.Redox biology · 2026Article
- Hormetic fasting extends Caenorhabditis elegans lifespan via H3K27 acetylation of lipid catabolism and antioxidant genes.Cell reports · 2026Article
- Protocol for automated analysis of oil red O-stained Caenorhabditis elegans using Redwormpix, a Python-based application.STAR protocols · 2026Article
- Intracellular lipid droplets positively regulate the dormancy of colorectal cancer cells depending on ELF1-ACSL5 signaling.Journal of experimental & clinical cancer research : CR · 2026Article
- Lipid Analysis in LiveBio-protocol · 2026Article
- Peroxisomes orchestrate metabolic flexibility and longevity via an interorganelle cascade.Nature aging · 2026Article
- Adaptive ER stress promotes mitochondrial remodelling and longevity through PERK-dependent MERCS assembly.Cell death and differentiation · 2026Article
- Size-Dependent Disruption of Lipid Metabolism by Polystyrene Micro- and Nanoplastics inToxics · 2026Article
- Article
- Hormetic nutrient stress promotes longevity by orchestrating histone acetylation on key lipid catabolism and antioxidant defense genes.bioRxiv : the preprint server for biology · 2025Article
- Postbiotic pA1cInternational journal of molecular sciences · 2025Article
- Method development for correlating lipid molecular information with anatomy in C. elegans.Scientific reports · 2025Article
- Article
- Black mulberry (Heliyon · 2025Article
- Functional analysis of conserved C. elegans bHLH family members uncovers lifespan control by a peptidergic hub neuron.PLoS biology · 2025Article
- Loss of the vitellogenins confers a fitness disadvantage but does not impact brood size inmicroPublication biology · 2025Article
- Gut microbiome speciesCurrent research in microbial sciences · 2025Article
- Microsporidia infection alters C. elegans lipid levels.PloS one · 2025Article
26 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
Caenorhabditis elegans is an exceptional model organism in which to study lipid metabolism and energy homeostasis. Many of its lipid genes are conserved in humans and are associated with metabolic syndrome or other diseases. Examination of lipid accumulation in this organism can be carried out by fixative dyes or label-free methods. Fixative stains like Nile red and oil red O are inexpensive, reliable ways to quantitatively measure lipid levels and to qualitatively observe lipid distribution across tissues, respectively. Moreover, these stains allow for high-throughput screening of various lipid metabolism genes and pathways. Additionally, their hydrophobic nature facilitates lipid solubility, reduces interaction with surrounding tissues, and prevents dissociation into the solvent. Though these methods are effective at examining general lipid content, they do not provide detailed information about the chemical composition and diversity of lipid deposits. For these purposes, label-free methods such as GC-MS and CARS microscopy are better suited, their costs notwithstanding.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.