ArticleJournal of visualized experiments : JoVE2018
Broth Microdilution In Vitro Screening: An Easy and Fast Method to Detect New Antifungal Compounds.
Article in Journal of visualized experiments : JoVE, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
13 citing papers in PubMed.
- Comparative Evaluation of Antimicrobial Activity of Dentin Disinfection Protocols Against MRSA andJournal of functional biomaterials · 2026Article
- Fungistatic activity and mechanism ofNarra J · 2025Article
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- Evaluation of the Cytotoxicity and Antifungal Efficacy ofAdvanced biomedical research · 2025Article
- Identification and Antimicrobial Potential of Marine Sponges (International journal of microbiology · 2025Article
- Plumbagin inhibits fungal growth, HMGB1/LOX-1 pathway and inflammatory factors inFrontiers in microbiology · 2024Article
- Biogenic synthesis of silver nanoparticle byFrontiers in microbiology · 2024Article
- Article
- Development of thermosensitive hydrogel of Amphotericin-B and Lactoferrin combination-loaded PLGA-PEG-PEI nanoparticles for potential eradication of ocular fungal infections:International journal of pharmaceutics: X · 2023Article
- Review
- Effect of various concentrations of common organic solvents on the growth and proliferation ability ofPeerJ · 2023Article
- Article
- Multi-omics Signature ofmSystems · 2019Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fungal infections have become an important medical condition in the last decades, but the number of available antifungal drugs is limited. In this scenario, the search for new antifungal drugs is necessary. The protocol reported here details a method to screen peptides for their antifungal properties. It is based on the broth microdilution susceptibility test from the Clinical and Laboratory Standards Institute (CLSI) M27-A3 guidelines with modifications to suit the research of antimicrobial peptides as potential new antifungals. This protocol describes a functional assay to evaluate the activity of antifungal compounds and may be easily modified to suit any particular class of molecules under investigation. Since the assays are performed in 96-well plates using small volumes, a large-scale screening can be completed in a short amount of time, especially if carried out in an automation setting. This procedure illustrates how a standardized and adjustable clinical protocol can help the bench-work pursuit of new molecules to improve the therapy of fungal diseases.
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Registered trials
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