ArticleJournal of visualized experiments : JoVE2018
Lens-free Video Microscopy for the Dynamic and Quantitative Analysis of Adherent Cell Culture.
Article in Journal of visualized experiments : JoVE, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
4 citing papers in PubMed.
- Extended Field of View and Resolution Enhancement in Lensless Digital Holography.Sensors (Basel, Switzerland) · 2026Article
- Detecting abnormal cell behaviors from dry mass time series.Scientific reports · 2024Article
- A novel feature for monitoring the enzymatic harvesting process of adherent cell cultures based on lens-free imaging.Scientific reports · 2022Article
- Quantitative phase imaging of adherent mammalian cells: a comparative study.Biomedical optics express · 2019Article
Corrections and comments
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Authors and funding
13 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Here, we demonstrate that lens-free video microscopy enables us to simultaneously capture the kinetics of thousands of cells directly inside the incubator and that it is possible to monitor and quantify single cells along several cell cycles. We describe the full protocol used to monitor and quantify a HeLa cell culture for 2.7 days. First, cell culture acquisition is performed with a lens-free video microscope, and then the data is analyzed following a four-step process: multi-wavelength holographic reconstruction, cell-tracking, cell segmentation and cell division detection algorithms. As a result, we show that it is possible to gather a dataset featuring more than 10,000 cell cycle tracks and more than 2 x 10
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