Evidence map›Paper›PMID 29553057›Full record

ArticleThe Journal of reproduction and development2018

Mitochondrial-related consequences of heat stress exposure during bovine oocyte maturation persist in early embryo development.

Rebecca R Payton, Louisa A Rispoli, Kimberly A Nagle, Cedric Gondro, Arnold M Saxton, Brynn H Voy, J Lannett Edwards

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of reproduction and development, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
23citing papers in PubMed, 1 pooled it
4.4field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

23 citing papers in PubMed, 1 synthesis or guideline pooled it, 49 citations in OpenAlex.

  1. Pooled it
  2. Article
  3. Transgenerational Impact of Environmental Change.Advances in experimental medicine and biology · 2026
    Review
  4. Article
  5. Article
  6. Review
  7. Review
  8. Article
  9. Review
  10. Article
  11. Review
  12. Article
  13. Review
  14. Article
  15. Article
  16. Article
  17. Article
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Rebecca R PaytonDepartment of Animal Science, The University of Tennessee, Institute of Agriculture, AgResearch, Knoxville, TN, USA.
Louisa A RispoliDepartment of Animal Science, The University of Tennessee, Institute of Agriculture, AgResearch, Knoxville, TN, USA.
Kimberly A NagleDepartment of Animal Science, The University of Tennessee, Institute of Agriculture, AgResearch, Knoxville, TN, USA.
Cedric GondroDepartment of Animal Science, Michigan State University, East Lansing, MI, USA.
Arnold M SaxtonDepartment of Animal Science, The University of Tennessee, Institute of Agriculture, AgResearch, Knoxville, TN, USA.
Brynn H VoyDepartment of Animal Science, The University of Tennessee, Institute of Agriculture, AgResearch, Knoxville, TN, USA.
J Lannett EdwardsDepartment of Animal Science, The University of Tennessee, Institute of Agriculture, AgResearch, Knoxville, TN, USA.
University of Tennessee at Knoxville · USMichigan State University · US

Funding

Tumor Immunology and Microenvironment Research ProgramP30CA068485 · NCI · VANDERBILT UNIVERSITY MEDICAL CENTER · PI MATTHEW J TYSKA · 1995 to 2026
$172.8M
NCI NIH HHS P30 CA068485
6 · The paper itself

Abstract

Hyperthermia during estrus has direct consequences on the maturing oocyte that carries over to the resultant embryo to compromise its ability to continue in development. Because early embryonic development is reliant upon maternal transcripts and other ooplasmic components, we examined impact of heat stress on bovine oocyte transcripts using microarray. Oocytes were matured at 38.5ºC for 24 h or 41.0ºC for the first 12 h of in vitro maturation; 38.5ºC thereafter. Transcriptome profile was performed on total (adenylated + deadenylated) RNA and polyadenylated mRNA populations. Heat stress exposure altered the abundance of several transcripts important for mitochondrial function. The extent to which transcript differences are coincident with functional changes was evaluated by examining reactive oxygen species, ATP content, and glutathione levels. Mitochondrial reactive oxygen species levels were increased by 6 h exposure to 41.0ºC while cytoplasmic levels were reduced compared to controls (P < 0.0001). Exposure to 41.0ºC for 12 h increased total and reduced glutathione levels in oocytes at 12 h but reduced them by 24 h (time × temperature P < 0.001). ATP content was higher in heat-stressed oocytes at 24 h (P < 0.0001). Heat-induced increases in ATP content of matured oocytes persisted in early cleavage-stage embryos (8- to 16-cell embryos; P < 0.05) but were no longer apparent in blastocysts (P > 0.05). Collectively, results indicate that direct exposure of maturing oocytes to heat stress may alter oocyte mitochondrial processes/function, which is inherited by the early embryo after fertilization.

Indexed as

Adenosine TriphosphateAnimalsCattleEmbryonic DevelopmentFemaleFertilization in VitroHot TemperatureIn Vitro Oocyte Maturation TechniquesMitochondriaOocytesOxidative PhosphorylationPregnancyReactive Oxygen SpeciesStress, PhysiologicalTranscriptomeAdenosine TriphosphateReactive Oxygen SpeciesATP contentGlutathioneHeat stressOxidative phosphorylationTranscriptome

Identifiers

PMID29553057
PMCPMC6021609
OpenAlexW2789752363

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.