ArticleCell2018
Transposase-DNA Complex Structures Reveal Mechanisms for Conjugative Transposition of Antibiotic Resistance.
Article in Cell, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 56 citations in OpenAlex.
- Mobile Genetic Elements as Central Drivers of Antimicrobial Resistance: Molecular Mechanisms, Evolutionary Ecology, One Health Implications and Control Strategies.Antibiotics (Basel, Switzerland) · 2026Review
- Reversing Antibiotic Resistance: Strategies From Adjuvants to Innovative Therapeutics.MicrobiologyOpen · 2026Review
- Antibiotic-degrading bacteria shape resistome dynamics and horizontal gene transfer potential in soils with contrasting properties.ISME communications · 2026Article
- Binding mode of Isoxazolyl Penicillins to a Class-A β-lactamase at ambient conditions.Communications chemistry · 2025Article
- Characteristics of the integration sites and module structures of the Tn916 and its family.Scientific reports · 2025Article
- Flavobacterium aestuarii sp. nov., a novel species isolated from the Pearl River Estuary.Antonie van Leeuwenhoek · 2025Article
- The Protective Effect of Omeprazole on Vancomycin Cytotoxicity in HK-2 Cells and Renal Injury in Rats.BioMed research international · 2025Article
- Transposition mechanism of ISAntimicrobial agents and chemotherapy · 2024Article
- Article
- Modularity and diversity of target selectors in Tn7 transposons.Molecular cell · 2023Article
- Landscape of mobile genetic elements and their antibiotic resistance cargo in prokaryotic genomes.Nucleic acids research · 2022Article
- Metagenomic Analysis Reveals A Possible Association Between Respiratory Infection and Periodontitis.Genomics, proteomics & bioinformatics · 2022Article
- Inner workings of RAG recombinase and its specialization for adaptive immunity.Current opinion in structural biology · 2021Review
- Multi-Omics Analysis Reveals the Mechanism Underlying the Edaphic Adaptation in Wild Barley at Evolution Slope (Tabigha).Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Article
- Sequence analysis of tyrosine recombinases allows annotation of mobile genetic elements in prokaryotic genomes.Molecular systems biology · 2021Article
- Genetic variation and function: revealing potential factors associated with microbial phenotypes.Biophysics reports · 2021Article
- Deep learning for HGT insertion sites recognition.BMC genomics · 2020Article
- Antimicrobial resistance in enteric bacteria: current state and next-generation solutions.Gut microbes · 2020Review
- Antibiotics in Food Chain: The Consequences for Antibiotic Resistance.Antibiotics (Basel, Switzerland) · 2020Review
- Nanopore sequencing reveals genomic map of CTX-M-type extended-spectrum β-lactamases carried by Escherichia coli strains isolated from blue mussels (Mytilus edulis) in Norway.BMC microbiology · 2020Article
Corrections and comments
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Authors and funding
10 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Conjugative transposition drives the emergence of multidrug resistance in diverse bacterial pathogens, yet the mechanisms are poorly characterized. The Tn1549 conjugative transposon propagates resistance to the antibiotic vancomycin used for severe drug-resistant infections. Here, we present four high-resolution structures of the conserved Y-transposase of Tn1549 complexed with circular transposon DNA intermediates. The structures reveal individual transposition steps and explain how specific DNA distortion and cleavage mechanisms enable DNA strand exchange with an absolute minimum homology requirement. This appears to uniquely allow Tn916-like conjugative transposons to bypass DNA homology and insert into diverse genomic sites, expanding gene transfer. We further uncover a structural regulatory mechanism that prevents premature cleavage of the transposon DNA before a suitable target DNA is found and generate a peptide antagonist that interferes with the transposase-DNA structure to block transposition. Our results reveal mechanistic principles of conjugative transposition that could help control the spread of antibiotic resistance genes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.