ArticleJournal of comparative physiology. B, Biochemical, systemic, and environmental physiology2018
The cryoprotectant system of Cope's gray treefrog, Dryophytes chrysoscelis: responses to cold acclimation, freezing, and thawing.
Article in Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 28 citations in OpenAlex.
- Evidence that local viscosity and NOX-dependent ROS increases render the tardigrade H. exemplaris resilient to extreme physical force.bioRxiv : the preprint server for biology · 2026Article
- Freeze-tolerant frogs accumulate cryoprotectants using photoperiod: A potential ecological trap.The Journal of animal ecology · 2025Article
- Winter Is Coming: Integrative Analysis of Cold Acclimation in a Freeze Tolerant Frog.Integrative organismal biology (Oxford, England) · 2025Article
- Insights into tail-belting by wild mice encourages fresh perspectives on physiological mechanisms that safeguard mammal tissues from freezing.Scientific reports · 2024Article
- Physiological responses to a changing winter climate in an early spring-breeding amphibian.Ecology and evolution · 2024Article
- Comparative transcriptomic analysis delineates adaptation strategies of Rana kukunoris toward cold stress on the Qinghai-Tibet Plateau.BMC genomics · 2024Article
- Investigation of seasonal changes in lipid synthesis and metabolism-related genes in the oviduct of Chinese brown frog (<em>Rana dybowskii</em>).European journal of histochemistry : EJH · 2023Article
- Seasonal variations in the intermediate metabolism in South American tree-frog Boana pulchella.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2022Article
- Metabolic responses of plasma to extreme environments in overwintering Tibetan frogs Nanorana parkeri: a metabolome integrated analysis.Frontiers in zoology · 2021Article
- Freeze tolerance and the underlying metabolite responses in the Xizang plateau frog, Nanorana parkeri.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2021Article
- Hepatic transcriptome of the freeze-tolerant Cope's gray treefrog, Dryophytes chrysoscelis: responses to cold acclimation and freezing.BMC genomics · 2020Article
- Overwintering adaptations and extreme freeze tolerance in a subarctic population of the wood frog, Rana sylvatica.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2019Review
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cope's gray treefrog (Dryophytes chrysoscelis) is one of few freeze-tolerant frogs that mobilize glycerol as a cryoprotectant, yet cold and freezing-induced accumulation of this and other osmolytes has received little attention in this species. This study investigated the development of freeze tolerance in D. chrysoscelis, analyzing the response of the cryoprotectant system to cold acclimation, freezing, and thawing. Glycerol production was low and unresponsive to acclimation temperature, or duration of acclimation to 5 °C, except for one cold-acclimated frog that presented elevated glycerol in plasma, liver, and skeletal muscle. Curiously, glycerol concentration in skeletal muscle was higher than that of plasma and liver, in both warm- and cold-acclimated frogs, suggesting glycerol synthesis in muscle. Urea concentration in plasma doubled in response to cold acclimation but did not change during freezing. Freezing induced hepatic glycogen catabolism and an increase in glycerol and glucose in several tissues, although the mobilization dynamics differed between these cryoprotectants, possibly as a result of different transport mechanisms. Although hepatic glucose mobilization was of considerable magnitude, glucose accumulation in peripheral tissues was low and was surpassed by that of glycerol and urea. The muscle production of glycerol and the cold-induced accumulation of urea imply a role for skeletal muscle metabolism in the mobilization of cryoprotective solutes in D. chrysoscelis. The cryoprotectant system of D. chrysoscelis is complex, highly variable, and unique, with glycerol, glucose, and likely urea serving as cryoprotectants.
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