ArticleBiochemical and biophysical research communications2018
Loquacious-PD removes phosphate inhibition of Dicer-2 processing of hairpin RNAs into siRNAs.
Article in Biochemical and biophysical research communications, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 9 citations in OpenAlex.
- RNAi-based pest control: Production, application and the fate of dsRNA.Frontiers in bioengineering and biotechnology · 2022Review
- Single-molecule analysis of processive double-stranded RNA cleavage by Drosophila Dicer-2.Nature communications · 2021Article
- RNA-binding protein Maca is crucial for gigantic male fertility factor gene expression, spermatogenesis, and male fertility, in Drosophila.PLoS genetics · 2021Article
- Article
- RNA methyltransferase BCDIN3D is crucial for female fertility and miRNA and mRNA profiles in Drosophila ovaries.PloS one · 2019Article
- LOTUS domain protein MARF1 binds CCR4-NOT deadenylase complex to post-transcriptionally regulate gene expression in oocytes.Nature communications · 2018Article
- An RNA-binding protein Blanks plays important roles in defining small RNA and mRNA profiles inHeliyon · 2018Article
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Authors and funding
1 author at 1 institution in 1 country.
Funding
Abstract
Drosophila Dicer-2 processes RNA substrates into short interfering RNAs (siRNAs). Loquacious-PD (Loqs-PD), a dsRNA-binding protein that associates with Dicer-2, is required for processing of a subset of RNA substrates including hairpin RNAs into siRNAs. Inorganic phosphate-a small molecule present in all cell types-inhibits Dicer-2 from processing precursor of microRNAs (pre-miRNAs), which are processed by Dicer-1. Whether or how Loqs-PD modulates the inhibitory effect of inorganic phosphate on Dicer-2 processing of RNA substrates is unknown. To address this question, I performed in vitro hairpin RNA processing assay with Dicer-2 in the presence or absence of Loqs-PD and/or inorganic phosphate. I found that inorganic phosphate inhibits Dicer-2 alone, but not Dicer-2 + Loqs-PD, from processing blunt-end hairpin RNAs into siRNAs. Thus, Loqs-PD removes the inhibitory effect of inorganic phosphate on Dicer-2 processing of blunt-end hairpin RNAs, allowing siRNA production in the presence of inorganic phosphate.
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Registered trials
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