ArticleScientific reports2018
A novel synthetic peptide microarray assay detects Chlamydia species-specific antibodies in animal and human sera.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers, 1 of them a synthesis that pooled it.
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Who cites it
22 citing papers in PubMed, 1 synthesis or guideline pooled it, 48 citations in OpenAlex.
- Current status and future directions ofFrontiers in veterinary science · 2026Pooled it
- Peptide Arrays as Tools for Unraveling Tumor Microenvironments and Drug Discovery in Oncology.Cells · 2026Review
- B-cell peptide epitopes as diagnostic targets for Q fever in sheep.Frontiers in microbiology · 2026Article
- Bead-Based Suspension Array Using Peptides as Antigens to Capture and Identify Serum Antibodies.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Identification of Schistosoma haematobium and Schistosoma mansoni linear B-cell epitopes with diagnostic potential using in silico immunoinformatic tools and peptide microarray technology.PLoS neglected tropical diseases · 2024Article
- Challenges in Chlamydial Serology: Insights from a Belgian and a Dutch Population Cohort.Microorganisms · 2024Article
- Genomic analysis of 61 Chlamydia psittaci strains reveals extensive divergence associated with host preference.BMC genomics · 2023Article
- Proof of concept for multiplex detection of antibodies against Chlamydia species in chicken serum using a bead-based suspension array with peptides as antigens.Veterinary research · 2023Article
- Applications of Peptide Microarrays in Autoantibody, Infection, and Cancer Detection.Methods in molecular biology (Clifton, N.J.) · 2023Review
- Article
- Experimental Chlamydia gallinacea infection in chickens does not protect against a subsequent experimental Chlamydia psittaci infection.Veterinary research · 2021Article
- Identification of a universal antigen epitope of influenza A virus using peptide microarray.BMC veterinary research · 2021Article
- Comparative Genome Analysis of 33Pathogens (Basel, Switzerland) · 2020Article
- In-depth proteomics approach reveals novel biomarkers of cardiac remodelling after myocardial infarction: An exploratory analysis.Journal of cellular and molecular medicine · 2020Article
- Use of meat juice and blood serum with a miniaturised protein microarray assay to develop a multi-parameter IgG screening test with high sample throughput potential for slaughtering pigs.BMC veterinary research · 2020Article
- Chlamydia-Induced Reactive Arthritis: Disappearing Entity or Lack of Research?Current rheumatology reports · 2019Review
- Multi-peptide ELISAs overcome cross-reactivity and inadequate sensitivity of conventional Chlamydia pneumoniae serology.Scientific reports · 2019Article
- Anti-chlamydia IgG and IgA are insufficient to prevent endometrial chlamydia infection in women, and increased anti-chlamydia IgG is associated with enhanced risk for incident infection.American journal of reproductive immunology (New York, N.Y. : 1989) · 2019Article
- Article
- Discovery of Human-Specific ImmunodominantmSphere · 2018Article
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Serological analysis of Chlamydia (C.) spp. infections is still mainly based on micro-immunofluorescence and ELISA. To overcome the limitations of conventional serology, we have designed a novel microarray carrying 52 synthetic peptides representing B-cell epitopes from immunodominant proteins of all 11 chlamydial species. The new assay has been validated using monospecific mouse hyperimmune sera. Subsequently, serum samples from cattle, sheep and humans with a known history of chlamydial infection were examined. For instance, the specific humoral response of sheep to treatment with a C. abortus vaccine has been visualized against a background of C. pecorum carriership. In samples from humans, dual infection with C. trachomatis and C. pneumoniae could be demonstrated. The experiments revealed that the peptide microarray assay was capable of simultaneously identifying specific antibodies to each Chlamydia spp. The actual assay represents an open platform test that can be complemented through future advances in Chlamydia proteome research. The concept of the highly parallel multi-antigen microarray proven in this study has the potential to enhance our understanding of antibody responses by defining not only a single quantitative response, but also the pattern of this response. The added value of using peptide antigens will consist in unprecedented serodiagnostic specificity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.