ArticleScientific reports2018
Environmental DNA filtration techniques affect recovered biodiversity.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
39 citing papers in PubMed, 168 citations in OpenAlex.
- Advancements in Technologies Targeting Horizontal Gene TransferRoutes to Control Drug Resistance Evolution.ACS bio & med chem Au · 2026Review
- Impact of sample processing method and volume on 16 S rRNA profiling of the urobiome.BMC microbiology · 2026Article
- Spatiotemporal Dynamics of Micropropagules in Seawater During the 2020 Green Tide Outbreak in the Southern Yellow Sea.Biology · 2026Article
- Advancing airborne eDNA sampling methods for monitoring diverse terrestrial vertebrate communities.Communications biology · 2026Article
- Horizontal Dispersal Limitation and Vertical Environmental Filtering Drive Ciliate Community Assembly in a Tibetan Plateau Deep Lake.Microorganisms · 2026Article
- From Sequences to Food Webs: DNA Metabarcoding Reshapes Fish Trophic Ecology.Animals : an open access journal from MDPI · 2026Review
- Survey of vulnerable Amazonian manatees using environmental DNA (eDNA): A method for survey in remote field settings.PloS one · 2026Article
- QuickConc: A Rapid, Efficient, and Power-Free eDNA Concentration Method With Cationic-Assisted Capture.Ecology and evolution · 2025Article
- Harnessing eDNA technology to identify fish diversity and distribution in the Kashi River, a tributary of the Ili River, Xinjiang, China.Scientific reports · 2025Article
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- Environment DNA Reveals Fish Diversity in a Canyon River within the Upper Pearl River Drainage.Animals : an open access journal from MDPI · 2024Article
- Advances in environmental DNA monitoring: standardization, automation, and emerging technologies in aquatic ecosystems.Science China. Life sciences · 2024Review
- Method for lysis and paper-based elution-free DNA extraction with colourimetric isothermal amplification.Scientific reports · 2024Article
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- Article
- Review
- Impact of filter material and holding time on spore sampling efficiency in water.Letters in applied microbiology · 2023Article
- Environmental DNA Assay for the Detection of the American Bullfrog (Animals : an open access journal from MDPI · 2023Article
- Article
- Quantitative PCR (qPCR) assay for the specific detection of the Chinese mystery snail (Cipangopaludina chinensis) in the UK.PloS one · 2023Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Freshwater metazoan biodiversity assessment using environmental DNA (eDNA) captured on filters offers new opportunities for water quality management. Filtering of water in the field is a logistical advantage compared to transport of water to the nearest lab, and thus, appropriate filter preservation becomes crucial for maximum DNA recovery. Here, the effect of four different filter preservation strategies, two filter types, and pre-filtration were evaluated by measuring metazoan diversity and community composition, using eDNA collected from a river and a lake ecosystem. The filters were preserved cold on ice, in ethanol, in lysis buffer and dry in silica gel. Our results show that filters preserved either dry or in lysis buffer give the most consistent community composition. In addition, mixed cellulose ester filters yield more consistent community composition than polyethersulfone filters, while the effect of pre-filtration remained ambiguous. Our study facilitates development of guidelines for aquatic community-level eDNA biomonitoring, and we advocate filtering in the field, using mixed cellulose ester filters and preserving the filters either dry or in lysis buffer.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.