Evidence map›Paper›PMID 29548718›Full record

ArticleBrazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]

Molecular characterization of feline calicivirus variants from multicat household and public animal shelter in Rio de Janeiro, Brazil.

Joylson de Jesus Pereira, Natasha Baumworcel, Júlia Monassa Fioretti, Cinthya Fonseca Domingues, Laís Fernandes de Moraes, Robson Dos Santos Souza Marinho, Maria Clara Rodrigues Vieira, Ana Maria Viana Pinto, Tatiana Xavier de Castro

Open access · hybridAbstract read
In one paragraph

Article in Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
0.7field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 13 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Joylson de Jesus PereiraUniversidade Federal Fluminense, Instituto Biomédico, Departamento de Microbiologia e Parasitologia, Niterói, RJ, Brazil. Electronic address: el.joy_j@yahoo.com.br.
Natasha BaumworcelUniversidade Federal Fluminense, Faculdade de Veterinária, Niterói, RJ, Brazil.
Júlia Monassa FiorettiInstituto Oswaldo Cruz - Fiocruz, Laboratório de Virologia Comparada e Ambiental, Rio de Janeiro, RJ, Brazil.
Cinthya Fonseca DominguesUniversidade Federal Fluminense, Instituto Biomédico, Departamento de Microbiologia e Parasitologia, Niterói, RJ, Brazil.
Laís Fernandes de MoraesUniversidade Federal Fluminense, Faculdade de Veterinária, Niterói, RJ, Brazil.
Robson Dos Santos Souza MarinhoUniversidade Federal Fluminense, Instituto Biomédico, Departamento de Microbiologia e Parasitologia, Niterói, RJ, Brazil.
Maria Clara Rodrigues VieiraUniversidade Federal Fluminense, Instituto Biomédico, Departamento de Microbiologia e Parasitologia, Niterói, RJ, Brazil; Universidade Federal Fluminense, Instituto de Biologia, Departamento de Biologia Celular e Molecular, Programa de Pós-graduação em Ciências e Biotecnologia, Niterói, RJ, Brazil.
Ana Maria Viana PintoUniversidade Federal Fluminense, Instituto Biomédico, Departamento de Microbiologia e Parasitologia, Niterói, RJ, Brazil.
Tatiana Xavier de CastroUniversidade Federal Fluminense, Instituto Biomédico, Departamento de Microbiologia e Parasitologia, Niterói, RJ, Brazil.
Universidade Federal Fluminense · BRFundação Oswaldo Cruz · BR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The aim of this study was to perform the molecular characterization of conserved and variable regions of feline calicivirus capsid genome in order to investigate the molecular diversity of variants in Brazilian cat population. Twenty-six conjunctival samples from cats living in five public short-term animal shelters and three multicat life-long households were analyzed. Fifteen cats had conjunctivitis, three had oral ulceration, eight had respiratory signs (cough, sneeze and nasal discharge) and nine were asymptomatic. Feline calicivirus were isolated in CRFK cells and characterized by reverse transcription PCR target to both conserved and variable regions of open reading frame 2. The amplicons obtained were sequenced. A phylogenetic analysis along with most of the prototypes available in GenBank database and an amino acid analysis were performed. Phylogenetic analysis based on both conserved and variable region revealed two clusters with an aLTR value of 1.00 and 0.98 respectively and the variants from this study belong to feline calicivirus genogroup I. No association between geographical distribution and/or clinical signs and clustering in phylogenetic tree was observed. The variants circulating in public short-term animal shelter demonstrated a high variability because of the relatively rapid turnover of carrier cats constantly introduced of multiple viruses into this location over time.

Indexed as

AnimalsBrazilCaliciviridae InfectionsCalicivirus, FelineCapsid ProteinsCat DiseasesCatsGenome, ViralOpen Reading FramesPetsPhylogenyCapsid ProteinsConjunctivitisFCV genogroupHouseholdMolecular characterizationShelter

Identifiers

PMID29548718
PMCPMC6175694
OpenAlexW2793819819

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.