ArticleTranslational oncology2018
Human Papillomavirus Type 16 Integration Analysis by Real-time PCR Assay in Associated Cancers.
Article in Translational oncology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
18 citing papers in PubMed, 27 citations in OpenAlex.
- Exploring the association between the lineages of human papillomavirus type 16 and viral physical status in the development of cervical cancer in Iran.Scientific reports · 2025Article
- Detection of partial deletions of the human papillomavirus 16 E6 gene in a subset of oropharyngeal and cervical cancers.Virus research · 2025Article
- The role of HR-HPV integration in the progression of premalignant lesions into different cancer types.Heliyon · 2024Review
- Case-control study: Unveiling human polyomaviruses and papillomavirus in Egyptian colorectal cancer patients.PloS one · 2024Article
- Incidence and association of high-risk HPVs and EBV in patients with advanced stages of colorectal cancer from Qatar.Human vaccines & immunotherapeutics · 2023Article
- Coinfection of HPVs Is Associated with Advanced Stage in Colorectal Cancer Patients from Qatar.Pathogens (Basel, Switzerland) · 2023Article
- Human Papillomaviruses-Related Cancers: An Update on the Presence and Prevention Strategies in the Middle East and North African Regions.Pathogens (Basel, Switzerland) · 2022Review
- Prevalence of Human Papillomavirus and Co-Infection with Epstein-Barr Virus in Oral and Oropharyngeal Squamous Cell Carcinomas.Asian Pacific journal of cancer prevention : APJCP · 2022Article
- Presence of Human Papillomavirus DNA in Malignant Neoplasia and Non-Malignant Breast Disease.Current issues in molecular biology · 2022Article
- Causes and Consequences of HPV Integration in Head and Neck Squamous Cell Carcinomas: State of the Art.Cancers · 2021Review
- Copresence of High-Risk Human Papillomaviruses and Epstein-Barr Virus in Colorectal Cancer: A Tissue Microarray and Molecular Study from Lebanon.International journal of molecular sciences · 2021Article
- Plasma Changes of Branched-Chain Amino Acid in Patients with Esophageal Cancer.Middle East journal of digestive diseases · 2021Article
- Co-presence of Epstein-Barr virus and high-risk human papillomaviruses in Syrian colorectal cancer samples.Human vaccines & immunotherapeutics · 2020Article
- Clinical Significance of Plasma Levels of Gluconeogenic Amino Acids in Esophageal Cancer Patients.Asian Pacific journal of cancer prevention : APJCP · 2020Article
- High prevalent human papillomavirus infections of the oral cavity of asymptomatic HIV-positive men.BMC infectious diseases · 2020Article
- Molecular evidence of human papillomaviruses in the retinoblastoma tumor.Virusdisease · 2019Article
- Whole genome sequencing reveals complexity in both HPV sequences present and HPV integrations in HPV-positive oropharyngeal squamous cell carcinomas.BMC cancer · 2019Article
- The Molecular Detection of Human Bocavirus (HBoV) in Colorectal Tissue with Malignant and Non-Malignant LesionsAsian Pacific journal of cancer prevention : APJCP · 2018Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human papillomavirus (HPV) is a common viral infection worldwide associated with a variety of cancers. The integration of the HPV genome in these patients causes chromosomal instability and triggers carcinogenesis. The aim of this study was to investigate the HPV-16 genome physical status in four major cancers related to HPV infection. Formalin-fixed paraffin-embedded blocks from our previous projects on head and neck, colorectal, penile, and cervical cancers were collected, and HPV-16-positive specimens were used for further analysis. The DNA extraction copy number of E2 and E7 genes was calculated by qualitative real-time PCR method. Serially diluted standards that were cloned in PUC57 plasmid were used. Standard curve and melting curve analysis was used for quantification. Of the 672 specimens studied, 76 (11.3%) were HPV-16 positive. We found that 35.6% (16/45) were integrated. Statistical analysis showed that there were significant correlations between integration of HPV-16 and cervical cancer end-stage carcinogenesis (P < .0001), episomal form, and ASCUS lesions (P = .045). Significant correlation in penile cancer patients was seen between the episomal form and high-grade cancer stage (P = .037). Integration is a major factor in the carcinogenesis mechanism of HPV and has different prevalence in various cancers with a higher rate in progression except in penile cancer.
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Registered trials
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