Evidence map›Paper›PMID 29546667›Full record

ArticleVirus genes2018

Proteomic analysis of A-549 cells infected with human adenovirus 40 by LC-MS.

Ana Carla Peixoto Guissoni, Célia Maria Almeida Soares, Kareem R Badr, Fabiola Sousa Ficcadori, Ana Flávia Alves Parente, Juliana Alves Parente, Lilian Cristina Baeza, Menira Souza, Divina das Dores de Paula Cardoso

Abstract read
PubMed Publisher
In one paragraph

Article in Virus genes, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.5field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
  2. The Role of Tricarboxylic Acid Cycle Metabolites in Viral Infections.Frontiers in cellular and infection microbiology · 2021
    Review
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Ana Carla Peixoto GuissoniHuman Virology Laboratory, Institute of Tropical Pathology and Public Health, Federal University of Goias, Rua 235, S/N, Sala 418, Setor Universitário, Goiania, Goias, 74605050, Brazil.
Célia Maria Almeida SoaresMolecular Biology Laboratory, Institute of Biological Sciences, Federal University of Goias, Goiania, Goias, Brazil.
Kareem R BadrHuman Virology Laboratory, Institute of Tropical Pathology and Public Health, Federal University of Goias, Rua 235, S/N, Sala 418, Setor Universitário, Goiania, Goias, 74605050, Brazil.
Fabiola Sousa FiccadoriHuman Virology Laboratory, Institute of Tropical Pathology and Public Health, Federal University of Goias, Rua 235, S/N, Sala 418, Setor Universitário, Goiania, Goias, 74605050, Brazil.
Ana Flávia Alves ParenteMolecular Biology Laboratory, Institute of Biological Sciences, Federal University of Goias, Goiania, Goias, Brazil.
Juliana Alves ParenteMolecular Biology Laboratory, Institute of Biological Sciences, Federal University of Goias, Goiania, Goias, Brazil.
Lilian Cristina BaezaMolecular Biology Laboratory, Institute of Biological Sciences, Federal University of Goias, Goiania, Goias, Brazil.
Menira SouzaHuman Virology Laboratory, Institute of Tropical Pathology and Public Health, Federal University of Goias, Rua 235, S/N, Sala 418, Setor Universitário, Goiania, Goias, 74605050, Brazil.
Divina das Dores de Paula CardosoHuman Virology Laboratory, Institute of Tropical Pathology and Public Health, Federal University of Goias, Rua 235, S/N, Sala 418, Setor Universitário, Goiania, Goias, 74605050, Brazil. dcardoso@ufg.br.ORCID http://orcid.org/0000-0003-0946-6168
Universidade Federal de Goiás · BR

Funding

Conselho Nacional de Desenvolvimento Científico e Tecnológico nº 031/ 2010Conselho Nacional de Desenvolvimento Científico e Tecnológico Process nº 563998 / 2010-5
6 · The paper itself

Abstract

Human Adenoviruses (HAdVs) are etiological agents of different syndromes such as gastroenteritis, cystitis, ocular, and respiratory diseases, and infection by these viruses may cause alterations in cellular homeostasis. The objective of the study was the proteomic analysis of A-549 cells infected with HAdV-40 using LC-MS. At 30 h of infection, the quantitative analysis revealed 336 differentially expressed proteins. From them, 206 were induced (up-regulated) and 130 were suppressed (down-regulated). The majority of up-regulated proteins were related to energy, cellular organization, stress response, and apoptosis pathways. It was observed alteration of cell metabolism with increase of the glycolytic pathway, β-oxidation, and respiratory chain. Also, the results suggest cytoskeleton reorganization and apoptosis induction. The data can improve knowledge about the replication of HAdV-40 in cell culture considering the proteins related to distinct metabolic pathways induced by viral infection in A-549 cells.

Indexed as

ProteomeAdenoviruses, HumanCell Line, TumorChild, PreschoolChromatography, LiquidHumansMass SpectrometryRespiratory MucosaProteomeA-549 cellsHuman adenovirusProteomic analyses

Identifiers

PMID29546667
OpenAlexW2792604017

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.