ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2018
Map-based cloning and characterization of Zea mays male sterility33 (ZmMs33) gene, encoding a glycerol-3-phosphate acyltransferase.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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33 citing papers in PubMed, 73 citations in OpenAlex.
- The OsRhoGAP2-OsRAC5 complex regulates rice seed germination via modulating aleurone layer development and α-amylase activity.Plant cell reports · 2026Article
- Redundant Glycerol-3-Phosphate Acyltransferases Regulate Thermo-Sensitive Genic Male Sterility in Rice.Plant biotechnology journal · 2026Article
- Convergent evolution of sex chromosomes in two palm species, Phoenix dactylifera and Kerriodoxa elegans.Annals of botany · 2025Article
- Decades' progress and prospects on maize functional genomics and molecular breeding.Science China. Life sciences · 2025Review
- Biochemical Insights into Lipid Remodeling in Wheat Anthers Under High-Temperature Stress.International journal of molecular sciences · 2025Article
- Key genes and molecular mechanisms responsible for male sterility revealed by transcriptome analysis in cotton.Plant cell reports · 2025Article
- Plastid-localized ZmENR1/ZmHAD1 complex ensures maize pollen and anther development through regulating lipid and ROS metabolism.Nature communications · 2024Article
- Genome-Wide Identification, Characterization, Evolutionary Analysis, and Expression Pattern of the GPAT Gene Family in Barley and Functional Analysis ofInternational journal of molecular sciences · 2024Article
- CRISPR/Cas9-based genome editing of 14 lipid metabolic genes reveals a sporopollenin metabolon ZmPKSB-ZmTKPR1-1/-2 required for pollen exine formation in maize.Plant biotechnology journal · 2024Article
- Beyond skin-deep: targeting the plant surface for crop improvement.Journal of experimental botany · 2023Review
- ZmRPN1 confers quantitative variation in pollen number and boosts hybrid seed production in maize.Plant biotechnology journal · 2023Article
- Triphasic regulation of ZmMs13 encoding an ABCG transporter is sequentially required for callose dissolution, pollen exine and anther cuticle formation in maize.Journal of advanced research · 2023Article
- A Systematic Investigation of Lipid Transfer Proteins Involved in Male Fertility and Other Biological Processes in Maize.International journal of molecular sciences · 2023Article
- Current insights and advances into plant male sterility: new precision breeding technology based on genome editing applications.Frontiers in plant science · 2023Review
- The ZmMYB84-ZmPKSB regulatory module controls male fertility through modulating anther cuticle-pollen exine trade-off in maize anthers.Plant biotechnology journal · 2022Article
- The Loss-Function of the Male Sterile GeneCells · 2022Article
- Review
- The characterization and candidate gene isolation for a novel male-sterile mutant ms40 in maize.Plant cell reports · 2021Article
- Genome editing in cereal crops: an overview.Transgenic research · 2021Review
- Article
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Authors and funding
12 authors at 2 institutions in 1 country.
Funding
Abstract
key messageMap-based cloning of maize ms33 gene showed that ZmMs33 encodes a sn-2 glycerol-3-phosphate acyltransferase, the ortholog of rice OsGPAT3, and it is essential for male fertility in maize. Genetic male sterility has been widely studied for its biological significance and commercial value in hybrid seed production. Although many male-sterile mutants have been identified in maize (Zea mays L.), it is likely that most genes that cause male sterility are unknown. Here, we report a recessive genetic male-sterile mutant, male sterility33 (ms33), which displays small, pale yellow anthers, and complete male sterility. Using a map-based cloning approach, maize GRMZM2G070304 was identified as the ms33 gene (ZmMs33). ZmMs33 encodes a novel sn-2 glycerol-3-phosphate acyltransferase (GPAT) in maize. A functional complementation experiment showed that GRMZM2G070304 can rescue the male-sterile phenotype of the ms33-6029 mutant. GRMZM2G070304 was further confirmed to be the ms33 gene via targeted knockouts induced by the clustered regularly interspersed short palindromic repeats (CRISPR)/Cas9 system. ZmMs33 is preferentially expressed in the immature anther from the quartet to early-vacuolate microspore stages and in root tissues at the fifth leaf growth stage. Phylogenetic analysis indicated that ZmMs33 and OsGPAT3 are evolutionarily conserved for anther and pollen development in monocot species. This study reveals that the monocot-specific GPAT3 protein plays an important role in male fertility in maize, and ZmMs33 and mutants in this gene may have value in maize male-sterile line breeding and hybrid seed production.
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