ArticleBioscience reports2018
Validation and application of a novel cholesterol efflux assay using immobilized liposomes as a substitute for cultured cells.
Article in Bioscience reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Homocysteine thiolactone affects paraoxonase 1 activity via altered paraoxonase 1 distribution on high-density lipoprotein particles.Bioscience reports · 2026Article
- Unlocking mRNA-driven CRISPR-Cas9 gene therapy via optimizing mRNA and the delivery vectors.Molecular therapy. Nucleic acids · 2025Review
- Reverse cholesterol transport: current assay methods, alterations with disease and response to therapeutic intervention.Frontiers in cardiovascular medicine · 2025Review
- Nanoparticle technology for mRNA: Delivery strategy, clinical application and developmental landscape.Theranostics · 2024Review
- Cell-free, high-density lipoprotein-specific phospholipid efflux assay predicts incident cardiovascular disease.The Journal of clinical investigation · 2023Article
- Improvement in bilirubin influence on cholesterol efflux capacity evaluation using the immobilized liposome-bound gel beads method.Bioscience reports · 2023Article
- Development and validation of novel automatable assay for cholesterol efflux capacity.Bioscience reports · 2023Article
- HDL Is Not Dead Yet.Biomedicines · 2022Review
- The nano delivery systems and applications of mRNA.European journal of medicinal chemistry · 2022Review
- Comparison of a novel cholesterol efflux assay using immobilized liposome-bound gel beads with the conventional method.Bioscience reports · 2020Article
- A Novel Cell-Free Fluorescent Assay for HDL Function: Low Apolipoprotein A1 Exchange Rate Associated with Increased Incident Cardiovascular Events.The journal of applied laboratory medicine · 2020Article
- Article
- Cholesterol efflux capacity assay using immobilized liposomes and apolipoprotein B-depleted serum.Bioscience reports · 2019Article
- Usefulness of apolipoprotein B-depleted serum in cholesterol efflux capacity assays using immobilized liposome-bound gel beads.Bioscience reports · 2019Article
Corrections and comments
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Authors and funding
12 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Estimation of the function as well as the amount of high-density lipoprotein (HDL) is required to predict the risk of cardiovascular disease development. Cholesterol efflux capacity (CEC) is the key metric for determining the antiatherosclerotic function of HDL. However, the assay methods currently used to calculate CEC are not ideal for clinical use as they require the culture of cells. In the present study, we developed a novel CEC assay using immobilized liposome-bound gel beads (ILGs), containing fluorescently labeled cholesterol, as a substitute for cultured cells. When apolipoprotein B-100 depleted serum, obtained by polyethylene glycol precipitation, was used as the cholesterol acceptors, the basic properties of this method, such as the available range of HDL-cholesterol, efflux temperature and time, and normalization parameters, indicate that this method is sufficient to estimate CEC. Furthermore, the CEC values obtained with this ILG method were also correlated with those obtained with a conventional method using THP-1 macrophages derived foam cells and
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