ArticlePLoS pathogens2018
The root-knot nematode effector MiPFN3 disrupts plant actin filaments and promotes parasitism.
Article in PLoS pathogens, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 62 citations in OpenAlex.
- A natural variation in rice susceptibility factor Rsf1 confers resistance to root-knot nematode.Nature communications · 2026Article
- Combating Root-Knot Nematodes (Plants (Basel, Switzerland) · 2025Review
- Article
- Counter-attack of biocontrol agents: Environmentally benign Approaches against Root-knot nematodes (Heliyon · 2023Review
- Biotechnological Tools to Elucidate the Mechanism of Plant and Nematode Interactions.Plants (Basel, Switzerland) · 2023Review
- Discovery of Novel Effector Protein Candidates Produced in the Dorsal Gland of Adult Female Root-Knot Nematodes.Molecular plant-microbe interactions : MPMI · 2023Article
- The Cytoskeleton in Plant Immunity: Dynamics, Regulation, and Function.International journal of molecular sciences · 2022Review
- Fungal Effectoromics: A World in Constant Evolution.International journal of molecular sciences · 2022Review
- Article
- Microbial Effectors: Key Determinants in Plant Health and Disease.Microorganisms · 2022Review
- Encoding, transmission, decoding, and specificity of calcium signals in plants.Journal of experimental botany · 2022Review
- An unconventionally secreted effector from the root knot nematode Meloidogyne incognita, Mi-ISC-1, promotes parasitism by disrupting salicylic acid biosynthesis in host plants.Molecular plant pathology · 2022Article
- Genome Expression Dynamics Reveal the Parasitism Regulatory Landscape of the Root-Knot NematodeGenes · 2021Article
- Effectors of Root-Knot Nematodes: An Arsenal for Successful Parasitism.Frontiers in plant science · 2021Review
- Comparative Analysis ofFrontiers in plant science · 2021Article
- A new esophageal gland transcriptome reveals signatures of large scale de novo effector birth in the root lesion nematode Pratylenchus penetrans.BMC genomics · 2020Article
- Analyses of the Root-Knot Nematode (Pathogens (Basel, Switzerland) · 2020Article
- Differential localization of Hessian fly candidate effectors in resistant and susceptible wheat plants.Plant direct · 2020Article
- Plant Proteins and Processes Targeted by Parasitic Nematode Effectors.Frontiers in plant science · 2019Review
- Battlefield Cytoskeleton: Turning the Tide on Plant Immunity.Molecular plant-microbe interactions : MPMI · 2019Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Root-knot nematodes secrete effectors that manipulate their host plant cells so that the nematode can successfully establish feeding sites and complete its lifecycle. The root-knot nematode feeding structures, their "giant cells," undergo extensive cytoskeletal remodeling. Previous cytological studies have shown the cytoplasmic actin within the feeding sites looks diffuse. In an effort to study root-knot nematode effectors that are involved in giant cell organogenesis, we have identified a nematode effector called MiPFN3 (Meloidogyne incognita Profilin 3). MiPFN3 is transcriptionally up-regulated in the juvenile stage of the nematode. In situ hybridization experiments showed that MiPFN3 transcribed in the nematode subventral glands, where it can be secreted by the nematode stylet into the plant. Moreover, Arabidopsis plants that heterologously expressed MiPFN3 were more susceptible to root-knot nematodes, indicating that MiPFN3 promotes nematode parasitism. Since profilin proteins can bind and sequester actin monomers, we investigated the function of MiPFN3 in relation to actin. Our results show that MiPFN3 suppressed the aberrant plant growth phenotype caused by the misexpression of reproductive actin (AtACT1) in transgenic plants. In addition, it disrupted actin polymerization in an in vitro assay, and it reduced the filamentous actin network when expressed in Arabidopsis protoplasts. Over a decade ago, cytological studies showed that the cytoplasmic actin within nematode giant cells looked fragmented. Here we provide the first evidence that the nematode is secreting an effector that has significant, direct effects on the plant's actin cytoskeleton.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.