ArticleeLife2018
A universal vector concept for a direct genotyping of transgenic organisms and a systematic creation of homozygous lines.
Article in eLife, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed, 19 citations in OpenAlex.
- Sixteen isotropic 3D fluorescence live-imaging datasets of gastrulation in the red flour beetle Tribolium castaneum (Coleoptera: Tenebrionidae).Scientific data · 2026Article
- Fifty high-content light sheet fluorescence microscopy datasets of Tribolium castaneum embryogenesis.Scientific data · 2025Article
- Long-term fluorescence live imaging of honeybee embryos using light sheet fluorescence microscopy and halocarbon-based liquids.Biology open · 2025Article
- A new suite of reporter vectors and a novel landing site survey system to study cis-regulatory elements in diverse insect species.Scientific reports · 2024Article
- Next generation marker-based vector concepts for rapid and unambiguous identification of single and double homozygous transgenic organisms.Biology open · 2023Article
- Article
- Generating and testing the efficacy of transgenic Cas9 in Tribolium castaneum.Insect molecular biology · 2022Article
- Review
- Patterning with clocks and genetic cascades: Segmentation and regionalization of vertebrate versus insect body plans.PLoS genetics · 2021Review
- A deterministic genotyping workflow reduces waste of transgenic individuals by two-thirds.Scientific reports · 2021Article
- Unexpected mutual regulation underlies paralogue functional diversification and promotes epithelial tissue maturation in Tribolium.Communications biology · 2020Article
- An ancestral apical brain region contributes to the central complex under the control ofeLife · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Diploid transgenic organisms are either hemi- or homozygous. Genetic assays are, therefore, required to identify the genotype. Our AGameOfClones vector concept uses two clearly distinguishable transformation markers embedded in interweaved, but incompatible Lox site pairs. Cre-mediated recombination leads to hemizygous individuals that carry only one marker. In the following generation, heterozygous descendants are identified by the presence of both markers and produce homozygous progeny that are selected by the lack of one marker. We prove our concept in
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.