Evidence map›Paper›PMID 29542839›Full record

ArticleThe Journal of eukaryotic microbiology2018

Detection of Weakly Expressed Trypanosoma cruzi Membrane Proteins Using High-Performance Probes.

Teresa Cruz-Bustos, Silvia N J Moreno, Roberto Docampo

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of eukaryotic microbiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
1.6field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 14 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Teresa Cruz-BustosCenter for Tropical and Emerging Global Diseases, University of Georgia, Athens, Georgia, 30602.
Silvia N J MorenoCenter for Tropical and Emerging Global Diseases, University of Georgia, Athens, Georgia, 30602.ORCID 0000-0002-2041-6295
Roberto DocampoCenter for Tropical and Emerging Global Diseases, University of Georgia, Athens, Georgia, 30602.ORCID 0000-0003-4229-8784
University of Georgia · US

Funding

Pathogenesis of Trypanosoma cruzi infectionR01AI107663 · NIAID · UNIVERSITY OF GEORGIA · PI DOCAMPO, ROBERTO · 2014 to 2017
$1.5M
Pathogenesis of Trypanosoma cruzi infectionR56AI107663 · NIAID · UNIVERSITY OF GEORGIA · PI DOCAMPO, ROBERTO · 2013 to 2013
$350k
NIAID NIH HHS R01 AI107663NIAID NIH HHS R56 AI107663
6 · The paper itself

Abstract

Membrane proteins in trypanosomatids are, in general, weakly expressed and confirmation of their subcellular localization frequently requires their overexpression with epitope tags. However, overexpression can lead to mislocalization of the probes. Viswanathan et al. (Nat. Methods, 2015, 12:568) described high performance tags for localization of weakly expressed proteins. We report here the use of these protein tags, named "spaghetti monster," for CRISPR/Cas9-mediated C-terminal endogenous tagging of Trypanosoma cruzi to localize two weakly expressed transient receptor potential channels to acidic compartments. The results indicate that this method will improve the detection of membrane proteins in T. cruzi.

Indexed as

Membrane ProteinsProtein TransportProtozoan ProteinsRecombinant Fusion ProteinsTrypanosoma cruziMembrane ProteinsProtozoan ProteinsRecombinant Fusion ProteinsAcidic compartmensgene tagginglysosomesspaghetti monster tagTrypanosoma cruzi

Identifiers

PMID29542839
PMCPMC6120783
OpenAlexW2791163035

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.