ArticleJournal of feline medicine and surgery2019
Detection of feline coronavirus mutations in paraffin-embedded tissues in cats with feline infectious peritonitis and controls.
Article in Journal of feline medicine and surgery, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 31 citations in OpenAlex.
- Longitudinal detection and genetic characterization of feline coronavirus in a captive cheetah population.The Journal of general virology · 2026Article
- Applying Supervised Machine Learning to Effusion Analysis for the Diagnosis of Feline Infectious Peritonitis.Bioengineering (Basel, Switzerland) · 2026Article
- Single-site mutation (M1058L) of feline infectious peritonitis virus (FIPV) spike protein in domestic cats of Northern Vietnam.Archives of virology · 2026Article
- Feline enteropathogens and molecular diagnostics: benefits, limitations and clinical applications.Journal of feline medicine and surgery · 2025Review
- Phylogenetic, clinical, pathological and epidemiological characterization of feline coronavirus infections in cats, in Istanbul.Frontiers in veterinary science · 2025Article
- Myocarditis in an FIP-Diseased Cat with FCoV M1058L Mutation: Clinical and Pathological Changes.Animals : an open access journal from MDPI · 2024Article
- Review
- An updated review of feline coronavirus: mind the two biotypes.Virus research · 2023Review
- 2022 AAFP/EveryCat Feline Infectious Peritonitis Diagnosis Guidelines.Journal of feline medicine and surgery · 2022Review
- Relationship between Uveal Inflammation and Viral Detection in 30 Cats with Feline Infectious Peritonitis.Pathogens (Basel, Switzerland) · 2022Article
- Detection of Feline Coronavirus Variants in Cats without Feline Infectious Peritonitis.Viruses · 2022Article
- Molecular epidemiology of type I and II feline coronavirus from cats with suspected feline infectious peritonitis in China between 2019 and 2021.Archives of virology · 2022Article
- Article
- Feline coronavirus with and without spike gene mutations detected by real-time RT-PCRs in cats with feline infectious peritonitis.Journal of feline medicine and surgery · 2020Article
- FCoV Viral Sequences of Systemically Infected Healthy Cats Lack Gene Mutations Previously Linked to the Development of FIP.Pathogens (Basel, Switzerland) · 2020Article
- Feline Infectious Peritonitis Virus Nsp5 Inhibits Type I Interferon Production by Cleaving NEMO at Multiple Sites.Viruses · 2019Article
- Review
- Article
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectivesThe amino acid substitutions M1058L and S1060A in the spike protein of feline coronavirus (FCoV) have been postulated to be responsible for the development of the pathogenic feline infectious peritonitis virus (FIPV), which causes feline infectious peritonitis (FIP). The aim of the following study was to investigate the presence of mutated virus in tissue samples of cats with and without FIP.
methodsThe study population consisted of 64 cats, 34 of which were diagnosed with FIP and 30 control cats. All cases underwent autopsy, histopathology and immunohistochemistry (IHC) for FCoV. Furthermore, a genotype-discriminating quantitative reverse transcriptase PCR (RT-qPCR) was performed on shavings of paraffin-embedded tissues to discriminate between cats with FIP and controls, and the sensitivity and specificity of this discriminating RT-qPCR were calculated using 95% confidence intervals (CIs).
resultsSpecificity of genotype-discriminating RT-qPCR was 100.0% (95% CI 88.4-100.0), and sensitivity was 70.6% (95% CI 52.5-84.9). In cats with FIP, 24/34 tested positive for FIPV. In samples of three control cats and in seven cats with FIP, FCoV was found, but genotyping was not possible owing to low FCoV RNA concentrations. Out of the positive samples, 23 showed the amino acid substitution M1058L in the spike protein and none the substitution S1060A. One sample in a cat with FIP revealed a mixed population of non-mutated FCoV and FIPV (mixed genotype). For one sample genotyping was not possible despite high viral load, and two samples were negative for FCoV. CONCLUSIONS AND RELEVANCE: As none of the control animals showed FCoV amino acid substitutions previously demonstrated in cats with FIP, it can be presumed that the substitution M1058L correlates with the presence of FIP. FCoV was detected in low concentration in tissues of control animals, confirming the ability of FCoV to spread systemically. The fact that no negative controls were included in the IHC protocol could potentially lead to an underestimation of the sensitivity of the RT-qPCR.
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