ReviewAdvances in experimental medicine and biology2018
CRISPR Knockouts in Ciona Embryos.
Review in Advances in experimental medicine and biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 28 citations in OpenAlex.
- A protocadherin mediates oral placode morphogenesis in the tunicate Ciona.Biology open · 2026Article
- Neuron-specific Agrin splicing by Nova RNA-binding proteins regulates conserved neuromuscular junction development in chordates.PLoS biology · 2025Article
- A gene regulatory network for specification and morphogenesis of a Mauthner Cell homolog in non-vertebrate chordates.Developmental biology · 2025Article
- CRISPR/Cas9 Protocols for Disrupting Gene Function in the Non-vertebrate Chordate Ciona.Integrative and comparative biology · 2024Review
- Specification and survival of post-metamorphic branchiomeric neurons in a non-vertebrate chordate.Development (Cambridge, England) · 2024Article
- A conserved RNA switch for acetylcholine receptor clustering at neuromuscular junctions in chordates.bioRxiv : the preprint server for biology · 2024Article
- Distribution of cionin, a cholecystokinin/gastrin family peptide, and its receptor in the central nervous system of Ciona intestinalis type A.Scientific reports · 2024Article
- Reaping the benefits of liquid handlers for high-throughput gene expression profiling in a marine model invertebrate.BMC biotechnology · 2024Article
- Loss of collagen gene expression in the notochord of the tailless tunicate Molgula occulta.Integrative and comparative biology · 2023Article
- Using CRISPR/Cas9 to identify genes required for mechanosensory neuron development and function.Biology open · 2023Article
- Specification and survival of post-metamorphic branchiomeric neurons in the hindbrain of a non-vertebrate chordate.bioRxiv : the preprint server for biology · 2023Article
- Ring Finger 149-Related Is an FGF/MAPK-Independent Regulator of Pharyngeal Muscle Fate Specification.International journal of molecular sciences · 2023Article
- Using CRISPR/Cas9 to identify genes required for mechanosensory neuron development and function.bioRxiv : the preprint server for biology · 2023Article
- Marine animal evolutionary developmental biology-Advances through technology development.Evolutionary applications · 2023Article
- Evolution of a chordate-specific mechanism for myoblast fusion.Science advances · 2022Article
- Pax3/7 regulates neural tube closure and patterning in a non-vertebrate chordate.Frontiers in cell and developmental biology · 2022Article
- CRISPR-Cas9 editing of non-coding genomic loci as a means of controlling gene expression in the sea urchin.Developmental biology · 2021Article
- Effect of Polycyclic Aromatic Hydrocarbons on Development of the AscidianInternational journal of environmental research and public health · 2020Article
- Effector gene expression underlying neuron subtype-specific traits in the Motor Ganglion of Ciona.Developmental biology · 2020Article
- TheFrontiers in cell and developmental biology · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
Clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 has emerged as a revolutionary tool for fast and efficient targeted gene knockouts and genome editing in almost any organism. The laboratory model tunicate Ciona is no exception. Here, we describe our latest protocol for the design, implementation, and evaluation of successful CRISPR/Cas9-mediated gene knockouts in somatic cells of electroporated Ciona embryos. Using commercially available reagents, publicly accessible plasmids, and free web-based software applications, any Ciona researcher can easily knock out any gene of interest in their favorite embryonic cell lineage.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.