ArticleAdvances in experimental medicine and biology2018
Purification of Fluorescent Labeled Cells from Dissociated Ciona Embryos.
Article in Advances in experimental medicine and biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Cell cycle-driven transcriptome maturation confers multilineage competence to cardiopharyngeal progenitors.The EMBO journal · 2025Article
- A gene regulatory network for specification and morphogenesis of a Mauthner Cell homolog in non-vertebrate chordates.Developmental biology · 2025Article
- A single oscillating proto-hypothalamic neuron gates taxis behavior in the primitive chordate Ciona.Current biology : CB · 2023Article
- A single oscillating proto-hypothalamic neuron gates taxis behavior in the primitive chordatebioRxiv : the preprint server for biology · 2023Article
- Ring Finger 149-Related Is an FGF/MAPK-Independent Regulator of Pharyngeal Muscle Fate Specification.International journal of molecular sciences · 2023Article
- Methods for cell isolation and analysis of the highly regenerative tunicateFrontiers in cell and developmental biology · 2023Article
- Onecut Regulates Core Components of the Molecular Machinery for Neurotransmission in Photoreceptor Differentiation.Frontiers in cell and developmental biology · 2021Article
- Simple Method to Quantify Protein Abundances from 1000 Cells.ACS omega · 2020Article
- Effector gene expression underlying neuron subtype-specific traits in the Motor Ganglion of Ciona.Developmental biology · 2020Article
- Regulation of Neurogenesis by FGF Signaling and Neurogenin in the Invertebrate ChordateFrontiers in cell and developmental biology · 2020Article
- Article
- A single-cell transcriptional roadmap for cardiopharyngeal fate diversification.Nature cell biology · 2019Article
- Single-cell transcriptome profiling of the Ciona larval brain.Developmental biology · 2019Article
- Initial characterization of Wnt-Tcf functions during Ciona heart development.Developmental biology · 2019Article
- A complete statistical model for calibration of RNA-seq counts using external spike-ins and maximum likelihood theory.PLoS computational biology · 2019Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Genome-wide studies in Ciona often require highly purified cell populations. In this methods chapter, we introduce multi-channel combinatorial fluorescence activated cells sorting (FACS) and magnetic-activated cell sorting (MACS) as two sensitive and efficient tools for isolating lineage-specific cell populations from dissociated Ciona embryos and larvae. We present isolation of trunk ventral cell (TVC) progeny as the test case most commonly used in our laboratory. These approaches may also be applied to purify other cell populations with the proper combination of tissue-specific reporters.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.