ArticlePlanta2018
RNA interference-based silencing of the alpha-amylase (amy1) gene in Aspergillus flavus decreases fungal growth and aflatoxin production in maize kernels.
Article in Planta, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 1 synthesis or guideline pooled it, 54 citations in OpenAlex.
- Resistance of Transgenic Maize Cultivars to Mycotoxin Production-Systematic Review and Meta-Analysis.Toxins · 2024Pooled it
- AI and Biotechnology to Combat Aflatoxins: Future Directions for Modern Technologies in Reducing Aflatoxin Risk.Toxins · 2025Review
- Review
- Host-Induced Gene Silencing of theToxins · 2024Article
- Article
- TheJournal of fungi (Basel, Switzerland) · 2024Article
- RNAi-Based Approaches to Control Mycotoxin Producers: Challenges and Perspectives.Journal of fungi (Basel, Switzerland) · 2024Review
- Molecular Characteristics and Functional Identification of a Key Alpha-Amylase-Encoding GeneInternational journal of molecular sciences · 2024Article
- The occurrence and management of fumonisin contamination across the food production and supply chains.Journal of advanced research · 2024Review
- Multiplexed Host-Induced Gene Silencing ofToxins · 2023Article
- Putative Core Transcription Factors Affecting Virulence inJournal of fungi (Basel, Switzerland) · 2023Article
- Targeting theFrontiers in plant science · 2023Article
- Cross-kingdom small RNA communication between plants and fungal phytopathogens-recent updates and prospects for future agriculture.RNA biology · 2023Review
- Advanced mycotoxin control and decontamination techniques in view of an increased aflatoxin risk in Europe due to climate change.Frontiers in microbiology · 2022Review
- Concepts and considerations for enhancing RNAi efficiency in phytopathogenic fungi for RNAi-based crop protection using nanocarrier-mediated dsRNA delivery systems.Frontiers in fungal biology · 2022Review
- Enzymatic degradation is an effective means to reduce aflatoxin contamination in maize.BMC biotechnology · 2021Article
- RNA Interference and CRISPR/Cas Gene Editing for Crop Improvement: Paradigm Shift towards Sustainable Agriculture.Plants (Basel, Switzerland) · 2021Review
- Host-Induced Gene Silencing of aFrontiers in plant science · 2021Article
- Comprehensive Transcriptome and Proteome Analyses Reveal the Modulation of Aflatoxin Production byFrontiers in microbiology · 2020Article
- Host Induced Gene Silencing TargetingFrontiers in microbiology · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
MAIN
conclusionExpressing an RNAi construct in maize kernels that targets the gene for alpha-amylase in Aspergillus flavus resulted in suppression of alpha-amylase (amy1) gene expression and decreased fungal growth during in situ infection resulting in decreased aflatoxin production. Aspergillus flavus is a saprophytic fungus and pathogen to several important food and feed crops, including maize. Once the fungus colonizes lipid-rich seed tissues, it has the potential to produce toxic secondary metabolites, the most dangerous of which is aflatoxin. The pre-harvest control of A. flavus contamination and aflatoxin production is an area of intense research, which includes breeding strategies, biological control, and the use of genetically-modified crops. Host-induced gene silencing, whereby the host crop produces siRNA molecules targeting crucial genes in the invading fungus and targeting the gene for degradation, has shown to be promising in its ability to inhibit fungal growth and decrease aflatoxin contamination. Here, we demonstrate that maize inbred B104 expressing an RNAi construct targeting the A. flavus alpha-amylase gene amy1 effectively reduces amy1 gene expression resulting in decreased fungal colonization and aflatoxin accumulation in kernels. This work contributes to the development of a promising technology for reducing the negative economic and health impacts of A. flavus growth and aflatoxin contamination in food and feed crops.
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Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.