ArticleThe open medicinal chemistry journal2018
Purified Mushroom Tyrosinase Induced Melanogenic Protein Expression in B16F10 Melanocytes: A Quantitative Densitometric Analysis.
Article in The open medicinal chemistry journal, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
2 citing papers in PubMed, 7 citations in OpenAlex.
- Additive Effect of a Combination ofPharmaceuticals (Basel, Switzerland) · 2020Article
- Modified Riceberry rice extract suppresses melanogenesis-associated cell differentiation through tyrosinase-mediated MITF downregulation on B16 cells andResearch in pharmaceutical sciences · 2020Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundHuman skin exists in a wide range of different colors and gradations, ranging from white to brown to black. This is due to the presence of a chemically inert and stable pigment known as melanin, which is produced deep inside the skin but is displayed as a mosaic at the surface of the body. METHODS & MATERIALS: In mammalian melanocytes, melanosome is a highly specialized organelle where melanin is synthesized. Melanin synthesis is controlled by tyrosinase, the vital enzyme in melanogenic pathway. The present investigation is based on the effect of purified tyrosinase of
resultsAfter the treatment of purified tyrosinase B16F10 melanocytes did not show any cytotoxic effect. Melanin content in B16F10 melanocytes was increased by purified tyrosinase in a dose-dependent manner. Quantitative western blot analysis revealed that cellular tyrosinase intensity was enhanced after treatment with purified tyrosinase for 48 hours, where the band intensity had a steady increase in the absorption of purified tyrosinase in B16F10 cells. The density analysis described increased absorption for 2 to 5 bands as 2.7, 3.7, 6.7 and 8.6% respectively. The bands in the comparative analysis of western blot were between the Rf value range (0.40-0.57) with maximum absorption of 3000 intensity curve at 32μg/mL, rather than higher concentration 64μg/mL, showing a decrease in the absorption.
conclusionIt is presumed that purified tyrosinase can be used as contestants for the treatment of vitiligous skin conditions.
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Registered trials
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