ArticleFrontiers in plant science2018
Optimized Method of Extracting Rice Chloroplast DNA for High-Quality Plastome Resequencing and
Article in Frontiers in plant science, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed, 43 citations in OpenAlex.
- Comprehensive analysis of chloroplast genome evolution in Poaceae: codon usage patterns, selection pressures, and phylogenomic relationships.BMC genomics · 2026Article
- Integrative chloroplast omics in citrus: computational insights into genomic structural adaptation and phylogenetic relationships from 35 species.Frontiers in plant science · 2026Article
- Article
- Chloroplast genomic insights into adaptive evolution and rapid radiation in the genus Passiflora (Passifloraceae).BMC plant biology · 2025Article
- Exploring Plastomic Resources inGenes · 2024Article
- Molecular Characterization and Phylogenetic Analysis of Centipedegrass [Current issues in molecular biology · 2024Article
- A novel in-situ-process technique constructs whole circular cpDNA library.Plant methods · 2024Article
- Insights intoForestry research · 2024Article
- Characterization of the complete chloroplast genome sequence ofMitochondrial DNA. Part B, Resources · 2024Article
- Comprehensive analysis of the chloroplast genome and phylogenetic relationships ofMitochondrial DNA. Part B, Resources · 2024Article
- Complete chloroplast genomes of six neotropical palm species, structural comparison, and evolutionary dynamic patterns.Scientific reports · 2023Article
- Development of a High-Quality/Yield Long-Read Sequencing-Adaptable DNA Extraction Method for Crop Seeds.Plants (Basel, Switzerland) · 2023Article
- Article
- Chloroplast genome sequencing based on genome skimming for identification of Eriobotryae Folium.BMC biotechnology · 2021Article
- Osj10gBTF3-Mediated Import of Chloroplast Protein Is Essential for Pollen Development in Rice.Frontiers in plant science · 2021Article
- Recent Advances in Carbon and Nitrogen Metabolism in C3 Plants.International journal of molecular sciences · 2020Review
- A Repertory of Rearrangements and the Loss of an Inverted Repeat Region in Passiflora Chloroplast Genomes.Genome biology and evolution · 2020Article
- A Natural Variation inThe Plant cell · 2020Article
- Functional Analysis of Rice Long-Chain Acyl-CoA Synthetase 9 (International journal of molecular sciences · 2020Article
- Can we use it? On the utility of de novo and reference-based assembly of Nanopore data for plant plastome sequencing.PloS one · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chloroplasts, which perform photosynthesis, are one of the most important organelles in green plants and algae. Chloroplasts maintain an independent genome that includes important genes encoding their photosynthetic machinery and various housekeeping functions. Owing to its non-recombinant nature, low mutation rates, and uniparental inheritance, the chloroplast genome (plastome) can give insights into plant evolution and ecology and in the development of biotechnological and breeding applications. However, efficient methods to obtain high-quality chloroplast DNA (cpDNA) are currently not available, impeding powerful sequencing and further functional genomics research. To investigate effects on rice chloroplast genome quality, we compared cpDNA extraction by three extraction protocols: liquid nitrogen coupled with sucrose density gradient centrifugation, high-salt buffer, and Percoll gradient centrifugation. The liquid nitrogen-sucrose gradient method gave a high yield of high-quality cpDNA with reliable purity. The cpDNA isolated by this technique was evaluated, resequenced, and assembled
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.