ArticleScientific reports2018
Reduced background autofluorescence for cell imaging using nanodiamonds and lanthanide chelates.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 64 citations in OpenAlex.
- Opportunities for Quantum Sensing in Epilepsy Diagnostics.Sensors (Basel, Switzerland) · 2026Review
- Imaging transzonal projections in the cumulus-oocyte complexes: challenges and solutions†.Biology of reproduction · 2025Article
- Diamond Chemistry: Advances and Perspectives.Angewandte Chemie (International ed. in English) · 2025Review
- A systematic study on the use of multifunctional nanodiamonds for neuritogenesis and super-resolution imaging.Biomaterials research · 2023Article
- Use of Magnetic Modulation of Nitrogen-Vacancy Center Fluorescence in Nanodiamonds for Quantitative Analysis of Nanoparticles in Organisms.ACS measurement science au · 2022Article
- Luminescent Metal Complexes as Emerging Tools for Lipid Imaging.Topics in current chemistry (Cham) · 2022Review
- Live-cell fluorescence spectral imaging as a data science challenge.Biophysical reviews · 2022Review
- Targeting cell surface glycans with lectin-coated fluorescent nanodiamonds.Nanoscale advances · 2022Article
- Mn-Doped AgZnInS/ZnS Nanocrystals (NCs): Effects of Zn Etching on the NC Optical Properties.Optical Materials · 2022Article
- Fluorophore Localization Determines the Results of Biodistribution of Core-Shell Nanocarriers.International journal of nanomedicine · 2022Article
- Review
- Surface Modification of Fluorescent Nanodiamonds for Biological Applications.Nanomaterials (Basel, Switzerland) · 2021Review
- Out-of-Phase Imaging after Optical Modulation (OPIOM) for Multiplexed Fluorescence Imaging Under Adverse Optical Conditions.Methods in molecular biology (Clifton, N.J.) · 2021Article
- A Medium-Throughput System for In Vitro Oxidative Stress Assessment in IPEC-J2 Cells.International journal of molecular sciences · 2020Article
- Intrinsic anti-Stokes emission in living HeLa cells.PloS one · 2020Article
- A Perspective on Fluorescent Nanodiamond Bioimaging.Small (Weinheim an der Bergstrasse, Germany) · 2019Review
- Electro-optic imaging enables efficient wide-field fluorescence lifetime microscopy.Nature communications · 2019Article
- Applications of Nanodiamonds in the Detection and Therapy of Infectious Diseases.Materials (Basel, Switzerland) · 2019Review
- Review Article: Synthesis, properties, and applications of fluorescent diamond particles.Journal of vacuum science and technology. B, Nanotechnology & microelectronics : materials, processing, measurement, & phenomena : JVST B · 2019Review
- Visualizing neuroinflammation with fluorescence and luminescent lanthanide-based in situ hybridization.Journal of neuroinflammation · 2019Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bio-imaging is a key technique in tracking and monitoring important biological processes and fundamental biomolecular interactions, however the interference of background autofluorescence with targeted fluorophores is problematic for many bio-imaging applications. This study reports on two novel methods for reducing interference with cellular autofluorescence for bio-imaging. The first method uses fluorescent nanodiamonds (FNDs), containing nitrogen vacancy centers. FNDs emit at near-infrared wavelengths typically higher than most cellular autofluorescence; and when appropriately functionalized, can be used for background-free imaging of targeted biomolecules. The second method uses europium-chelating tags with long fluorescence lifetimes. These europium-chelating tags enhance background-free imaging due to the short fluorescent lifetimes of cellular autofluorescence. In this study, we used both methods to target E-selectin, a transmembrane glycoprotein that is activated by inflammation, to demonstrate background-free fluorescent staining in fixed endothelial cells. Our findings indicate that both FND and Europium based staining can improve fluorescent bio-imaging capabilities by reducing competition with cellular autofluorescence. 30 nm nanodiamonds coated with the E-selectin antibody was found to enable the most sensitive detective of E-selectin in inflamed cells, with a 40-fold increase in intensity detected.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.