ArticleScientific reports2018
Rapid histopathological imaging of skin and breast cancer surgical specimens using immersion microscopy with ultraviolet surface excitation.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
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Who cites it
57 citing papers in PubMed, 84 citations in OpenAlex.
- Diagnostic accuracy of two-photon fluorescence microscopy in Mohs surgery of basal cell carcinomas.Journal of the American Academy of Dermatology · 2026Article
- Ultraviolet Sensing-Guided Biomedical Systems: From Label-Free Imaging to Dosimetry and Therapy Feedback.Biosensors · 2026Review
- Semantic Segmentation for Pixel-Wise Visualization of Breast Cancer in Deep Ultraviolet-Excited Fluorescence Images.Acta histochemica et cytochemica · 2026Article
- Effective transfer of tumor annotations from hematoxylin and eosin to fluorescence images of breast and lung tissues.Journal of biomedical optics · 2026Article
- High area and volumetric throughput parallel two-photon fluorescence microscopy using silicon photomultiplier arrays.Photonics research · 2025Article
- H&E Style Translation Using CycleGAN for Deep Ultraviolet-Excitation Fluorescence Images of Pancreatic Endoscopic Ultrasound-Fine Needle Aspiration/Biopsy Toward Slide-Free Rapid Pathology.Acta histochemica et cytochemica · 2025Article
- Autofluorescence Virtual Staining System for H&E Histology and Multiplex Immunofluorescence Applied to Immuno-Oncology Biomarkers in Lung Cancer.Cancer research communications · 2025Article
- Perspective on the use of fluorescence molecular imaging for peripheral and deepJournal of biomedical optics · 2025Review
- Single color digital H&E staining with In-and-Out Net.Computerized medical imaging and graphics : the official journal of the Computerized Medical Imaging Society · 2024Article
- Metabolic light absorption, scattering, and emission (MetaLASE) microscopy.Science advances · 2024Article
- Rapid full-color serial sectioning tomography with speckle illumination and ultraviolet excitation.Npj imaging · 2024Article
- Quantitative and comparative assessment of dyes and protocols for rapid ex vivo microscopy of fresh tissues.Scientific reports · 2024Article
- NerveTracker: a Python-based software toolkit for visualizing and tracking groups of nerve fibers in serial block-face microscopy with ultraviolet surface excitation images.Journal of biomedical optics · 2024Article
- Piston-based specimen holder for rapid surgical and biopsy specimen imaging.Biomedical optics express · 2024Article
- Current Landscape of Advanced Imaging Tools for Pathology Diagnostics.Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc · 2024Review
- Structured illumination microscopy for cancer identification in diagnostic breast biopsies.PloS one · 2024Article
- Visualization of Gastric Adenocarcinoma Lymph Node Metastases by Microscopy with Ultraviolet Surface Excitation.Sovremennye tekhnologii v meditsine · 2024Article
- Deep learning-enabled realistic virtual histology with ultraviolet photoacoustic remote sensing microscopy.Nature communications · 2023Article
- Comprehensive Surface Histology of Fresh Resection Margins With Rapid Open-Top Light-Sheet (OTLS) Microscopy.IEEE transactions on bio-medical engineering · 2023Article
- Deep ultraviolet fluorescence microscopy of three-dimensional structures in the mouse brain.Scientific reports · 2023Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
Rapid histopathological evaluation of fresh, unfixed human tissue using optical sectioning microscopy would have applications to intraoperative surgical margin assessment. Microscopy with ultraviolet surface excitation (MUSE) is a low-cost optical sectioning technique using ultraviolet illumination which limits fluorescence excitation to the specimen surface. In this paper, we characterize MUSE using high incident angle, water immersion illumination to improve sectioning. Propidium iodide is used as a nuclear stain and eosin yellow as a counterstain. Histologic features of specimens using MUSE, nonlinear microscopy (NLM) and conventional hematoxylin and eosin (H&E) histology were evaluated by pathologists to assess potential application in Mohs surgery for skin cancer and lumpectomy for breast cancer. MUSE images of basal cell carcinoma showed high correspondence with frozen section H&E histology, suggesting that MUSE may be applicable to Mohs surgery. However, correspondence in breast tissue between MUSE and paraffin embedded H&E histology was limited due to the thicker optical sectioning in MUSE, suggesting that further development is needed for breast surgical applications. We further demonstrate that the transverse image resolution of MUSE is limited by the optical sectioning thickness and use co-registered NLM to quantify the improvement in MUSE optical sectioning from high incident angle water immersion illumination.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.